Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart Lung and Blood Vessel Disease, Beijing, P.R. China.
Sci Rep. 2017 Jan 11;7:40584. doi: 10.1038/srep40584.
We examined the effect of hydroxysafflor yellow A (HSYA) on the inflammatory response to strike-induced acute soft tissue injury in rats. Soft tissue injury was induced in rat leg muscles using a strike hammer, followed by intraperitoneal administration of HSYA at 16, 32, or 64 mg/kg. After 24 h, the rats were anaesthetized, blood and muscle samples were taken. Plasma levels of interleukin (IL)-6, IL-1β, and tumour necrosis factor (TNF)-αwere measured by enzyme-linked immunosorbent assay. Total RNA and protein were isolated from muscle tissue to determine the mRNA levels of IL-6, IL-1β, TNF-α, vascular cell adhesion molecule (VCAM)-1, and intercellular adhesion molecule (ICAM)-1, and the protein level of phosphorylated p38 mitogen-activated protein kinase (MAPK). Nuclear factor (NF)-κB expression was determined by muscle histopathology and immunohistochemistry. HSYA attenuated pathologic changes instrike-induced soft tissue inflammation. Treatment with HSYA also alleviated strike-induced increases in TNF-α, IL-1β, IL-6, VCAM-1, and ICAM-1mRNA levels and inhibited the increased activation of NF-κB and phosphorylation of p38 MAPK in muscle tissue. These findings suggest that HSYA effectively inhibits strike-induced inflammatory signal transduction in rats.
我们研究了羟基红花黄色素 A（HSYA）对打击诱导的急性软组织损伤大鼠炎症反应的影响。使用打击锤在大鼠腿部肌肉中诱导软组织损伤，然后腹膜内给予 HSYA 16、32 或 64mg/kg。24 小时后，麻醉大鼠，取血和肌肉样本。通过酶联免疫吸附试验测量血浆中白细胞介素（IL）-6、IL-1β和肿瘤坏死因子（TNF）-α的水平。从肌肉组织中分离总 RNA 和蛋白质，以确定 IL-6、IL-1β、TNF-α、血管细胞黏附分子（VCAM）-1 和细胞间黏附分子（ICAM）-1 的 mRNA 水平，以及磷酸化 p38 丝裂原活化蛋白激酶（p38 MAPK）的蛋白水平。通过肌肉组织病理学和免疫组织化学测定核因子（NF）-κB 的表达。HSYA 减轻了打击诱导的软组织炎症的病理变化。HSYA 治疗还减轻了打击引起的 TNF-α、IL-1β、IL-6、VCAM-1 和 ICAM-1mRNA 水平的增加，并抑制了肌肉组织中 NF-κB 的增加激活和 p38 MAPK 的磷酸化。这些发现表明 HSYA 可有效抑制大鼠打击诱导的炎症信号转导。
