羟基红花黄色素A对博莱霉素诱导的大鼠肺部炎症和纤维化的保护作用。
Protective effect of hydroxysafflor yellow A on bleomycin- induced pulmonary inflammation and fibrosis in rats.
作者信息
Jin Ming, Wang Lin, Wu Yan, Zang Bao-Xia, Tan Li
机构信息
Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, China.
出版信息
Chin J Integr Med. 2018 Jan;24(1):32-39. doi: 10.1007/s11655-017-2094-z. Epub 2018 Jan 3.
OBJECTIVE
To observe the effect of hydroxysafflor yellow A (HSYA), an active ingredient of a traditional Chinese herbal medicine Carthamus tinctorius L., on lung inflflammation and pulmonary fibrosis induced by bleomycin (BLM) in rats.
METHODS
Animals were divided into 6 groups including normal group, model group, three HSYA groups and dexamethasone (DXM) group. Three doses of HSYA (35.6, 53.3, and 80.0 mg•kg•day) were intraperitoneally (i.p.) injected in rats for 3 weeks after BLM administration and DXM was used as the positive control (n=8 or 12). Arterial blood gas was assayed and morphological changes were observed. Lung mRNA expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and some cytokines in lung tissue were detected by real-time polymerase chain reaction. Nuclear factor-κB p65 or α-smooth muscle actin (α-SMA) protein distribution in rat lung tissue was observed by immunohistochemistry.
RESULTS
On the 7th day after BLM administration, lung tissue showed serious inflammation. Treatment with HSYA or DXM ameliorated lung inflammation. After treatment with HSYA or DXM, oxygen partial pressure (PaO) increased (HSYA 80.0 mg•kg, P<0.01) and CO partial pressure (PaCO) decreased (HSYA 53.3, 80.0 mg•kg, P<0.05). Moreover, the mRNA expression of TNF-α, IL-1β, and IL-6; and the number of NF-κB p65 positive cells was lower in HSYA 53.3 and 80.0 mg•kg groups than those in the model group (all P<0.05). Twenty-one days after BLM administration, HSYA or DXM treatment ameliorated fibrosis, increased PaO2 (HSYA 53.3, 80.0 mg•kg-1, P<0.01), and decreased PaCO2 (53.3 and 80.0 mg•kg-1, P<0.05). Further, the mRNA expression of TGF-β1, α-SMA, and collagen I as well as the number of α-SMA positive cells increased in the model group and HSYA can attenuate these changes (53.3, 80.0 mg•kg, P<0.05). Hematoxylin and eosin and Masson's trichrome staining indicated that the fibrosis and collagen deposition were ameliorated in HSYA groups (53.3, 80.0 mg•kg, P<0.05).
CONCLUSION
HSYA could alleviate acute lung inflflammation and chronic pulmonary fibrosis induced by BLM in rats.
目的
观察中药红花的有效成分羟基红花黄色素A（HSYA）对博莱霉素（BLM）诱导的大鼠肺部炎症和肺纤维化的影响。
方法
将动物分为6组，包括正常组、模型组、3个HSYA组和地塞米松（DXM）组。BLM给药后3周，对大鼠腹腔注射3种剂量的HSYA（35.6、53.3和80.0mg•kg•天），持续3周，DXM作为阳性对照（n = 8或12）。检测动脉血气并观察形态学变化。通过实时聚合酶链反应检测肺组织中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6及一些细胞因子的肺mRNA表达。通过免疫组织化学观察大鼠肺组织中核因子-κB p65或α-平滑肌肌动蛋白（α-SMA）蛋白分布。
结果
BLM给药后第7天，肺组织出现严重炎症。HSYA或DXM治疗可减轻肺部炎症。HSYA或DXM治疗后，氧分压（PaO）升高（HSYA 80.0mg•kg，P<0.01），二氧化碳分压（PaCO）降低（HSYA 53.3、80.0mg•kg，P<0.05）。此外，HSYA 53.3和80.0mg•kg组中TNF-α、IL-1β和IL-6的mRNA表达以及NF-κB p65阳性细胞数均低于模型组（均P<0.05）。BLM给药21天后，HSYA或DXM治疗可减轻纤维化，提高PaO2（HSYA 53.3、80.0mg•kg-1，P<0.01），降低PaCO2（53.3和80.0mg•kg-1，P<0.05）。此外，模型组中转化生长因子-β1、α-SMA和I型胶原的mRNA表达以及α-SMA阳性细胞数增加，HSYA可减轻这些变化（53.3、80.0mg•kg，P<0.05）。苏木精-伊红染色和Masson三色染色表明HSYA组（53.3、80.0mg•kg，P<0.05）的纤维化和胶原沉积减轻。
结论
HSYA可减轻BLM诱导的大鼠急性肺部炎症和慢性肺纤维化。
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