[利用大插入片段基因组BAC克隆对鸟类Z和W染色体的拟常染色体及相邻区域进行的研究]

[Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones].

作者信息

Blagoveshchenskiĭ I Iu, Sazanova A L, Stekol'nikova V A, Fomichev K A, Barkova O Iu, Romanov M N, Sazanov A A

出版信息

Genetika. 2011 Mar;47(3):312-9.

Abstract

To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region.

摘要

为了研究鸟类Z和W染色体上的拟常染色体区域及相邻区域，我们使用了来自基因组文库的7个BAC克隆，作为家鸡和日本鹌鹑性染色体拟常染色体区域（PAR）近端边界附近ATP5A1基因不同配子同源物片段的DNA探针。通过使用W特异性探针进行荧光原位杂交，确定了BAC克隆TAM31 - b100C09、TAM31 - b99N01、TAM31 - b27P16和TAM31 - b95L18在家鸡和日本鹌鹑Z染色体短臂（区域Zp23 - p22）中的定位，以及BAC克隆CHORI - 261 - CH46G16、CHORI - 261 - CH33F10和CHORI - 261 - CH64F22在这些物种的W染色体上和Z染色体短臂（区域Zp23 - p22）中的定位。BAC克隆在Z和W染色体上定位的差异可能是由位于拟常染色体区域之外的不同性染色体核苷酸序列的差异所解释的。