Pakharukova N A, Pastushkova L Kh, Trifonova O P, Moshkovskiĭ S A, Larina I M
Fiziol Cheloveka. 2011 Mar-Apr;37(2):77-85.
For analysis of inter-individual variability in low-molecular serum subproteome proteome profiles of healthy men at the age of 20-30 years (36 subjects), 30-40 years (11 subjects) and 40-50 years (11 subjects) were obtained. Serum samples were fractionated on magnetic beads MB WCX using ClinProt robot prior to mass-spectrometry based profiling. Mass-spectra were obtained with time-of-flight mass-spectrometer Autoflex III ("Bruker Daltonics") in automatic mode. It was shown that low-molecular serum subproteome of healthy humans was characterized by significant inter-individual variability. 21% of all peaks in proteome profiles had coefficient of variation more than 50% and 29% of all peaks had low dispersion (CV < 30%).Therefore majority of peaks in proteome profile were peaks with moderate inter-individual variability (CV from 30% to 50%). Fragments of high-molecular kininogen, inter-alpha-trypsin inhibitor, complement components C3 and C4a, apolipoprotein CI, platelet factor IV, beta2-microglobulin and cystatin C showed wide variation among examined groups of healthy men. Dispersion of high-molecular kininogen, inter-alpha-trypsin inhibitor, apolipoproteins AII and CIII peaks increased with age.
为分析20 - 30岁健康男性（36名受试者）、30 - 40岁健康男性（11名受试者）和40 - 50岁健康男性（11名受试者）低分子血清亚蛋白质组蛋白质谱的个体间变异性，采集了血清样本。在基于质谱的分析之前，使用ClinProt机器人在磁珠MB WCX上对血清样本进行分级分离。使用飞行时间质谱仪Autoflex III（“布鲁克道尔顿公司”）以自动模式获得质谱图。结果表明，健康人的低分子血清亚蛋白质组具有显著的个体间变异性。蛋白质谱中所有峰的21%变异系数超过50%，所有峰的29%离散度较低（CV < 30%）。因此，蛋白质谱中的大多数峰是个体间变异性中等的峰（CV为30%至50%）。高分子量激肽原、α-胰蛋白酶抑制剂、补体成分C3和C4a、载脂蛋白CI、血小板因子IV、β2-微球蛋白和胱抑素C的片段在健康男性受试组中表现出广泛差异。高分子量激肽原、α-胰蛋白酶抑制剂、载脂蛋白AII和CIII峰的离散度随年龄增加。
