Valeeva O A, Pastushkov L Kh, Pakharukova N A, Dobrokhotov I V, Larina I M
Fiziol Cheloveka. 2011 May-Jun;37(3):98-102.
Human proteome is very plastic, it changes under the influence of various biological factors. It is of big interest to find out how specific factors of an environment, including a long-term isolation affect on urine proteome. The study was conducted during the experiment with 105-day isolation. In the present investigation we collected urine samples from 6 healthy volunteers (26-41 years old). The physical activity, daily rhythms and diet were controlled. Urine samples were fractionated on magnetic beads MB-HIC C8 (MB - hydrophobic-interaction chromatography) with ClinProt robot (Bruker Daltonics) prior to MALDI-TOF mass spectrometer analysis with Autoflex III TOF/TOF (Bruker Daltonics), working in a positive linear mode. 117 peaks have been obtained in each spectrum of urine. We have shown that even during isolation and under controlled conditions of life a high variability of urine proteome of healthy personas (36 protein MC-peaks in the urine, on average) are revealed.
人类蛋白质组具有很强的可塑性，它会在各种生物因素的影响下发生变化。弄清楚包括长期隔离在内的特定环境因素如何影响尿液蛋白质组是非常有意义的。该研究是在为期105天的隔离实验期间进行的。在本研究中，我们收集了6名健康志愿者（26 - 41岁）的尿液样本。对身体活动、日常节律和饮食进行了控制。在使用Autoflex III TOF/TOF（布鲁克道尔顿公司）以正线性模式工作的基质辅助激光解吸电离飞行时间质谱仪分析之前，先用ClinProt机器人（布鲁克道尔顿公司）在磁珠MB - HIC C8（MB - 疏水相互作用色谱）上对尿液样本进行分级分离。在每个尿液光谱中获得了117个峰。我们已经表明，即使在隔离期间以及在受控的生活条件下，健康人的尿液蛋白质组也显示出高度变异性（平均尿液中有36个蛋白质MC峰）。
