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新月柄杆菌螺旋形态突变体的特征分析

Characterization of a Helical Morphological Mutant of Caulobacter crescentus.

作者信息

Markiewicz Z, Dziechciarz K, Kwiatkowski Z

出版信息

Acta Microbiol Pol. 1986;35(1-2):5-13.

PMID:21542389
Abstract

Screening of poorly growing colonies following EMS treatment of Caulobacter crescentus culture revealed several mutants with altered cell morphology. One of these (CB15-UW.5) grew as elongated, helically twisted cells which were more susceptible to autolysis than the wild type. The mutant cells were found to have higher transglycosylase activity, this being reflected by shorter chain length of their murein. A second difference between the murein of the mutant and the parent was lower trimer content in the former, although in both cases the degree of crosslinkage was similar.

摘要

对经甲基磺酸乙酯(EMS)处理的新月柄杆菌培养物中生长不良的菌落进行筛选,发现了几个细胞形态发生改变的突变体。其中一个(CB15-UW.5)长成细长的螺旋状扭曲细胞,比野生型更容易自溶。发现突变体细胞具有更高的转糖基酶活性,这反映在其胞壁质的链长较短。突变体和亲本的胞壁质之间的第二个差异是前者的三聚体含量较低,尽管在两种情况下交联程度相似。

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