Markiewicz Z, Kwiatkowski Z
Acta Microbiol Pol. 1985;34(1):5-14.
Caulobacter crescentus was grown in complex medium supplemented with low (0.05%) concentration of glycine, a component of the murein peptide side chains of this bacterium. Murein synthesized in the presence of glycine was poorly crosslinked and the rate of its synthesis was slowed down compared to the control cells. The glycine-grown cells were considerably more sensitive to the chelating agent EDTA and Tris buffer than the control cells and also lysed faster when incubated with beta-lactam antibiotics. No changes in phospholipid composition in the presence of glycine were observed and the outer membrane protein composition of the glycine-grown cells was altered only in the amount of 130 000 protein which forms the surface array of C. crescentus. The effects of glycine can thus be tentatively put down to the reduced crosslinkage of murein synthesized in its presence.
新月柄杆菌在添加了低浓度（0.05%）甘氨酸的复合培养基中生长，甘氨酸是该细菌胞壁肽侧链的一个组成部分。与对照细胞相比，在甘氨酸存在下合成的胞壁质交联程度较差，其合成速率也减慢。与对照细胞相比，在甘氨酸中生长的细胞对螯合剂乙二胺四乙酸（EDTA）和 Tris 缓冲液更为敏感，并且在用β-内酰胺抗生素孵育时裂解得更快。在甘氨酸存在下未观察到磷脂组成的变化，在甘氨酸中生长的细胞的外膜蛋白组成仅在形成新月柄杆菌表面阵列的 130000 蛋白的量上有所改变。因此，甘氨酸的作用可以暂时归因于在其存在下合成的胞壁质交联减少。
