Laboratory of Cell-Death Control BioTechnology, Department of Life Sciences, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Shobara, Hiroshima, Japan.
Oncol Res. 2011;19(5):203-16. doi: 10.3727/096504011x12970940207805.
Fullerene [C60] included in polyethylene glycol (PEG) at a composing ratio of 1:350 w/w was examined for anticancer effects upon photodynamic therapy (PDT). Human connective tissue-derived fibrosarcoma cells HT1080 were decreased for a viability of 50% or 30%, by 3-h administration with PEG-fullerene [C60] at 50 or 100 ppm fullerene [C60] equivalent, respectively, subsequent rinsing out and irradiation with visible light (400-600 nm, 140 J/cm2: 450-fold as intense as in average outdoor), whereas the same tissue type-derived normal fibroblastic cells DUMS16 retained a viability of 93% or 85% under the same conditions. Anticancer effects were dependent on PEG-fullerene [C60] concentrations and irradiation doses, and scarcely exerted by PEG-fullerene [C60] alone, irradiation alone, or by fullerene [C60]-free PEG combined with irradiation, suggesting that the active principle may be fullerene [C60] as small as 0.0028 wt% versus the whole compound. Irradiation with PEG-fullerene [C60] occurred in intracellular DNA fragmentation according to TUNEL assay, and produced reactive oxygen species (ROS) such as hydroperoxides and peroxyl radicals or superoxide anion radicals in HT1080 cells as demonstrated by CDCFH-DA assay or nitroblue tetrazolium assay, respectively. Thus, PEG-fullerene [C60] is expected to be applied to anticancer PDT with scarce side effects on normal cells.
将富勒烯[C60]以 1:350 w/w 的组成比包含在聚乙二醇（PEG）中，用于光动力疗法（PDT）的抗癌作用研究。人结缔组织衍生的纤维肉瘤细胞 HT1080，在 3 小时内用 50 或 100ppm 富勒烯[C60]等效的 PEG-富勒烯[C60]处理后，细胞活力分别降低了 50%或 30%，随后用可见光（400-600nm，140J/cm2：强度是平均室外光照的 450 倍）照射并冲洗，但在相同条件下，同一组织类型衍生的正常成纤维细胞 DUMS16 的活力保持在 93%或 85%。抗癌作用取决于 PEG-富勒烯[C60]浓度和照射剂量，PEG-富勒烯[C60]单独、单独照射或不含富勒烯[C60]的 PEG 与照射结合几乎没有作用，表明活性物质可能是 0.0028wt%（相对于整个化合物）的富勒烯[C60]。根据 TUNEL 测定，用 PEG-富勒烯[C60]照射会导致细胞内 DNA 片段化，并在 HT1080 细胞中产生活性氧物质（ROS），如氢过氧化物和过氧自由基或超氧阴离子自由基，分别通过 CDCFH-DA 测定或硝基四唑蓝测定证实。因此，PEG-富勒烯[C60]有望应用于抗癌 PDT，对正常细胞的副作用较小。
