Li Qian, Liu Chenguang, Li Hongguang
J Nanosci Nanotechnol. 2016 Jun;16(6):5592-7. doi: 10.1166/jnn.2016.11717.
The production of ROS in mitochondria plays critical role in photodynamic therapy (PDT). The aim of this study was to investigate whether fullerene-based PDT can induce generation of additional endogenous ROS in mitochondria. Chitosan oligosaccharide grafted fullerene conjugate (CS-C60) was synthesized as a model water-soluble fullerene. The relationship among photodynamic cytotoxicity, intracellular ROS and CS-C60 amount demonstrated that low dose fullerene could induce generation of endogenous ROS in human malignant melanoma (A375) cells. Laser scanning microscope (LSM) image shows that considerable amount of endogenous ROS was generated in mitochondria even CS-C60 could not localize into mitochondria. Assay with rotenone shows that PDT-induced endogenous ROS was generated via electron transport chain (ETC).
线粒体中活性氧（ROS）的产生在光动力疗法（PDT）中起着关键作用。本研究的目的是调查基于富勒烯的光动力疗法是否能诱导线粒体中额外内源性ROS的产生。合成了壳寡糖接枝富勒烯共轭物（CS-C60）作为一种水溶性富勒烯模型。光动力细胞毒性、细胞内ROS与CS-C60含量之间的关系表明，低剂量富勒烯可诱导人恶性黑色素瘤（A375）细胞产生内源性ROS。激光扫描显微镜（LSM）图像显示，即使CS-C60不能定位于线粒体中，线粒体中也会产生大量内源性ROS。鱼藤酮检测表明，光动力疗法诱导产生的内源性ROS是通过电子传递链（ETC）产生的。
