Mechant E, De Marez T, Vroman G, Hermann O, Legrand G, Misonne J F, Bulcke R
Ghent University, Faculty of Bioscience Engineering, Dept. Plant Production, Weed Science Unit, Coupure Links 653, BE-9000 Gent, Belgium.
Commun Agric Appl Biol Sci. 2010;75(2):53-9.
Chenopodium album L. (fat-hen) with a Ser264-Gly mutation is resistant to photosystem II-inhibiting herbicides like the triazinone metamitron, a key herbicide in sugar beet. In recent years, this resistant biotype may cause unsatisfactory weed control in Belgian sugar beet. However, the dimension of the problem was yet unknown. Therefore, a survey was conducted in 2008 covering the whole Belgian sugar beet area. In randomly selected fields, C. album plants surviving weed control were counted and sampled. First, the number of surviving plants was used to estimate the prevalence of fields with unsatisfactory control and to classify the surveyed fields. Then, the share of the resistant biotype in each field was determined with cleaved amplified polymorphic sequence-analysis (CAPS-analysis) on sampled leaves. Finally, all results were visualised on the map of Belgium. Twenty percent of the fields had more than 500 surviving plants per hectare and were thus classified as fields with unsatisfactory C. album control. The resistant biotype was present in 95% of these fields and even in 74% of the sampled fields with good weed control. No pattern was found during mapping. These results indicate that the metamitron-resistant biotype has spread over the whole sugar beet area but that it is not (yet) causing severe problems in every field. To get a more accurate estimation of the portion of resistant plants in the field and the effect of herbicide treatment on this biotype, an elaborate survey will be conducted in 2010 on fields that have both untreated and treated plots installed.
具有Ser264 - Gly突变的藜（肥猪草）对抑制光系统II的除草剂具有抗性，如三嗪酮类除草剂苯嗪草酮，这是甜菜中的一种关键除草剂。近年来，这种抗性生物型可能导致比利时甜菜田杂草防除效果不佳。然而，该问题的严重程度尚不清楚。因此，2008年对比利时整个甜菜种植区进行了一次调查。在随机选择的田块中，统计并采集了在杂草防除后存活的藜属植物。首先，利用存活植株数量来估计防除效果不佳田块的比例，并对调查田块进行分类。然后，通过对采集叶片进行酶切扩增多态性序列分析（CAPS分析）来确定每个田块中抗性生物型的比例。最后，将所有结果绘制在比利时地图上。20%的田块每公顷有超过500株存活植株，因此被归类为藜属植物防除效果不佳的田块。在这些田块中，95%存在抗性生物型，甚至在杂草防除效果良好的采样田块中，74%也存在抗性生物型。在绘制地图过程中未发现规律。这些结果表明，抗苯嗪草酮生物型已扩散至整个甜菜种植区，但尚未在每个田块都造成严重问题。为了更准确地估计田间抗性植株的比例以及除草剂处理对这种生物型的影响，2010年将在设置了未处理和已处理小区的田块上进行详细调查。
