Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul, Korea.
J Agric Food Chem. 2011 Jun 8;59(11):6271-6. doi: 10.1021/jf2001537. Epub 2011 May 9.
The effects of riboflavin photosensitization on the oxidative stability of oil-in-water (O/W) emulsions were determined using lipid hydroperoxides and headspace volatile analyses. The influences of a metal chelator, sodium azide, and superoxide dismutase (SOD) on oxidation pathways were tested to gain a better understanding of the role of transition metals, singlet oxygen, and superoxide anion, respectively. Emulsions with riboflavin and visible light irradiation had significantly higher lipid hydroperoxides and volatiles (p < 0.05) as compared to samples without light irradiation or riboflavin. The addition of ethylenediammetetraacetic acid (EDTA) decreased the formation of lipid hydroperoxides, hexanal, 2-heptenal, and 1-octen-3-ol in a concentration-dependent manner. Sodium azide, a singlet oxygen physical quencher, only inhibited the formation of 2-heptenal and 1-octen-3-ol. Overall, photosensitized riboflavin participated in both type I and type II pathways in O/W emulsions, and these pathways enhance the prooxidant activity of metals through their ability to produce lipid hydroperoxides and superoxide anion.
采用脂质氢过氧化物和顶空挥发性分析方法，研究了核黄素敏化作用对水包油（O/W）乳液氧化稳定性的影响。通过测试金属螯合剂叠氮化钠和超氧化物歧化酶（SOD）对氧化途径的影响，以更好地了解过渡金属、单线态氧和超氧阴离子的作用。与未经光照或核黄素处理的样品相比，含有核黄素并经可见光照射的乳液具有更高的脂质氢过氧化物和挥发性物质（p < 0.05）。乙二胺四乙酸（EDTA）的添加以浓度依赖的方式减少了脂质氢过氧化物、己醛、2-庚醛和 1-辛烯-3-醇的形成。作为单线态氧物理猝灭剂的叠氮化钠仅抑制了 2-庚醛和 1-辛烯-3-醇的形成。总之，光致敏核黄素在 O/W 乳液中参与了 I 型和 II 型途径，这些途径通过产生脂质氢过氧化物和超氧阴离子增强了金属的促氧化剂活性。
