苜蓿中华根瘤菌 1021 中 2-氨基乙基膦酸盐降解途径的遗传和生化特性研究。
Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021.
机构信息
Institute for Genomic Biology, Howard Hughes Medical Institute University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
出版信息
J Biol Chem. 2011 Jun 24;286(25):22283-90. doi: 10.1074/jbc.M111.237735. Epub 2011 May 4.
Abstract
A variety of microorganisms have the ability to use phosphonic acids as sole sources of phosphorus. Here, a novel pathway for degradation of 2-aminoethylphosphonate in the bacterium Sinorhizobium meliloti 1021 is proposed based on the analysis of the genome sequence. Gene deletion experiments confirmed the involvement of the locus containing phnW, phnA, and phnY genes in the conversion of 2-aminoethylphosphonate to inorganic phosphate. Biochemical studies of the recombinant PhnY and PhnA proteins verified their roles as phosphonoacetaldehyde dehydrogenase and phosphonoacetate hydrolase, respectively. This pathway is likely not limited to S. meliloti as suggested by the presence of homologous gene clusters in other bacterial genomes.
摘要
多种微生物具有将膦酸作为唯一磷源的能力。在这里，基于对细菌 Sinorhizobium meliloti 1021 基因组序列的分析，提出了一种新型的 2-氨基乙基膦酸盐降解途径。基因缺失实验证实，包含 phnW、phnA 和 phnY 基因的基因座参与了 2-氨基乙基膦酸盐向无机磷酸盐的转化。对重组 PhnY 和 PhnA 蛋白的生化研究证实了它们分别作为膦酰基乙醛脱氢酶和膦酰基乙酸水解酶的作用。该途径可能不仅限于 S. meliloti，因为在其他细菌基因组中存在同源基因簇。
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