Department of Microbiology and Molecular Genetics, School of Medicine, Medical Sciences B240, University of California, Irvine, CA 92697-4025, USA.
J Virol. 2011 Jul;85(13):6809-13. doi: 10.1128/JVI.00679-11. Epub 2011 May 4.
Arginine methylation can regulate protein import and export and can modulate protein interactions. Herpes simplex virus 1 (HSV-1) ICP27 is a shuttling protein involved in viral mRNA export. We previously reported that ICP27 is methylated on three arginines within its RGG box and that arginine methylation regulates ICP27 export and its interaction with SRPK1 and Aly/REF. Here, we report that ICP27 was efficiently imported into the nucleus when hypomethylated as determined by Fluorescence Recovery After Photobleaching (FRAP). Furthermore, coimmunoprecipitation of ICP27 with β-importin was not significantly affected by ICP27 hypomethylation. Thus, ICP27 import does not appear to be regulated by arginine methylation.
精氨酸甲基化可以调节蛋白质的输入和输出，并可以调节蛋白质相互作用。单纯疱疹病毒 1（HSV-1）ICP27 是一种穿梭蛋白，参与病毒 mRNA 的输出。我们之前报道过，ICP27 的 RGG 盒内的三个精氨酸被甲基化，精氨酸甲基化调节 ICP27 的输出及其与 SRPK1 和 Aly/REF 的相互作用。在这里，我们报告说，通过荧光恢复后漂白（FRAP）测定，当 ICP27 去甲基化时，它可以有效地被导入细胞核。此外，ICP27 与β-importin 的共免疫沉淀不受 ICP27 去甲基化的显著影响。因此，ICP27 的输入似乎不受精氨酸甲基化的调节。
