Martins Nadia Helena, Meza Andreia Navarro, Santos Camila Ramos, de Giuseppe Priscila Oliveira, Murakami Mario Tyago
National Laboratory for Biosciences, National Center for Research in Energy and Materials, Campinas, Brazil.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):618-22. doi: 10.1107/S1744309111010414. Epub 2011 Apr 28.
Purine nucleoside phosphorylase (PNP; EC 2.4.2.1) is a key enzyme of the purine-salvage pathway. Its ability to transfer glycosyl residues to acceptor bases is of great biotechnological interest owing to its potential application in the synthesis of nucleoside analogues used in the treatment of antiviral infections and in anticancer chemotherapy. Although hexameric PNPs are prevalent in prokaryotes, some microorganisms, such as Bacillus subtilis, present both hexameric and trimeric PNPs. The hexameric PNP from B. subtilis strain 168, named BsPNP233, was cloned, expressed and crystallized. Crystals belonging to different space groups (P32(1), P2(1)2(1)2(1), P6(3)22 and H32) were grown in distinct conditions with pH values ranging from 4.2 to 10.5. The crystals diffracted to maximum resolutions ranging from 2.65 to 1.70 Å.
嘌呤核苷磷酸化酶（PNP；EC 2.4.2.1）是嘌呤补救途径的关键酶。由于其在用于治疗抗病毒感染和抗癌化疗的核苷类似物合成中的潜在应用，它将糖基残基转移至受体碱基的能力具有重大的生物技术意义。尽管六聚体PNP在原核生物中普遍存在，但一些微生物，如枯草芽孢杆菌，同时存在六聚体和三聚体PNP。来自枯草芽孢杆菌168菌株的六聚体PNP，命名为BsPNP233，被克隆、表达并结晶。属于不同空间群（P32(1)、P2(1)2(1)2(1)、P6(3)22和H32）的晶体在pH值范围为4.2至10.5的不同条件下生长。这些晶体的衍射分辨率最高可达2.65至1.70 Å。
