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Crystallization and preliminary X-ray studies of purine nucleoside phosphorylase from Cellulomonas sp.

作者信息

Bzowska A, Tebbe J, Luić M, Wielgus-Kutrowska B, Schröder W, Shugar D, Saenger W, Koellner G

机构信息

Department of Biophysics, Institute of Experimental Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.

出版信息

Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):1061-3. doi: 10.1107/s0907444998004120.

DOI:10.1107/s0907444998004120
PMID:9757137
Abstract

The commercially available enzyme purine nucleoside phosphorylase (PNP) from Cellulomonas sp. was purified by ion--exchange chromatography, partially sequenced and crystallized in two different crystal forms using the hanging-drop vapour-diffusion technique. Crystal form A grows as polyeders and/or cubes in the cubic space group P4232 with unit-cell dimension a = 162.5 A. Crystal form B appears as thick plates in the space group P212121 with unit-cell dimensions a = 63.2, b = 108.3 and c = 117.4 A. Both crystal forms contain three monomers (one trimer) in the asymmetric unit.

摘要

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