进口马达在跨膜段插入线粒体 TIM23 复合物中的作用。
Role of the import motor in insertion of transmembrane segments by the mitochondrial TIM23 complex.
机构信息
Institute for Physiological Chemistry and Centre for Integrated Protein Science, Ludwig-Maximilians University, Butenandtstrasse 5, 81377 Munich, Germany.
出版信息
EMBO Rep. 2011 Jun;12(6):542-8. doi: 10.1038/embor.2011.72. Epub 2011 May 6.
Abstract
The TIM23 complex mediates translocation of proteins across, and their lateral insertion into, the mitochondrial inner membrane. Translocation of proteins requires both the membrane-embedded core of the complex and its ATP-dependent import motor. Insertion of some proteins, however, occurs in the absence of ATP, questioning the need for the import motor during lateral insertion. We show here that the import motor associates with laterally inserted proteins even when its ATPase activity is not required. Furthermore, our results suggest a role for the import motor in lateral insertion. Thus, the import motor is involved in ATP-dependent translocation and ATP-independent lateral insertion.
摘要
TIM23 复合物介导蛋白质穿过线粒体内膜的易位及其侧向插入。蛋白质的易位需要复合物的膜嵌入核心及其 ATP 依赖性导入马达。然而，一些蛋白质的插入发生在没有 ATP 的情况下，这质疑了在侧向插入过程中是否需要导入马达。我们在这里表明，即使在不需要其 ATP 酶活性的情况下，导入马达也会与侧向插入的蛋白质结合。此外，我们的结果表明导入马达在侧向插入中起作用。因此，导入马达参与 ATP 依赖性易位和 ATP 非依赖性侧向插入。
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本文引用的文献
1
Mitochondrial protein import: from proteomics to functional mechanisms.线粒体蛋白导入：从蛋白质组学到功能机制。
Nat Rev Mol Cell Biol. 2010 Sep;11(9):655-67. doi: 10.1038/nrm2959.
2
Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting.不同形式的线粒体 TOM-TIM 超复合体决定了前体蛋白分拣的信号依赖状态。
Mol Cell Biol. 2010 Jan;30(1):307-18. doi: 10.1128/MCB.00749-09.
3
Multiple pathways for mitochondrial protein traffic.线粒体蛋白质运输的多种途径。
Biol Chem. 2009 Aug;390(8):723-30. doi: 10.1515/BC.2009.087.
4
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5
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6
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7
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8
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10
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