Okura Hisako, Toft Nils, Pozzato Nicola, Tondo Annalucia, Nielsen Søren Saxmose
Department of Large Animal Sciences, Faculty of Life Sciences, University of Copenhagen, Grønnegårdsvej 8, 1870 Frederiksberg C, Denmark.
Vet Med Int. 2011 Apr 13;2011:152687. doi: 10.4061/2011/152687.
Presence of Mycobacterium avium subsp. paratuberculosis (MAP) in beef has been reported as a public health concern because asymptomatically infected cattle may contain MAP in tissues that are used for human consumption. Associations between MAP carcasses contamination and animal characteristics such as age, breed, production type, and carcass classification were assessed. Cheek muscles from 501 carcasses were sampled cross-sectionally at a Danish abattoir and tested for presence of viable MAP and MAP DNA by bacterial culture and IS900 realtime PCR, respectively. Cheek muscle tissues from carcasses of two dairy cows were positive by culture whereas 4% of the animals were estimated with ≥10 CFU/gram muscle based on realtime PCR. Age was found to be associated with carcass contamination with MAP. The observed viable MAP prevalence in beef carcasses was low. However, detection of MAP and MAP DNA in muscle tissues suggested that bacteremia occurred in slaughtered cattle.
有报告称牛肉中存在副结核分枝杆菌（MAP）是一个公共卫生问题，因为无症状感染的牛的用于人类食用的组织中可能含有MAP。评估了MAP对胴体的污染与动物特征（如年龄、品种、生产类型和胴体分类）之间的关联。在丹麦一家屠宰场对501具胴体的脸颊肌肉进行横断面采样，分别通过细菌培养和IS900实时PCR检测活MAP和MAP DNA的存在情况。两头奶牛胴体的脸颊肌肉组织培养呈阳性，而基于实时PCR估计4%的动物每克肌肉中MAP≥10 CFU。发现年龄与MAP对胴体的污染有关。在牛肉胴体中观察到的活MAP流行率较低。然而，在肌肉组织中检测到MAP和MAP DNA表明屠宰牛发生了菌血症。
