商品家禽育种场中滑液支原体诊断血清学检测的比较研究
Comparative Study of Serological Tests for Mycoplasma synoviae Diagnosis in Commercial Poultry Breeders.
作者信息
Luciano R L, Cardoso A L S P, Stoppa G F Z, Kanashiro A M I, de Castro A G M, Tessari E N C
机构信息
Centro Avançado de Pesquisa Tecnológica do Agronegócio Avícola, Instituto Biológico, Rua Bezerra Paes, 2278, Jardim Belém, 13.690-000 Descalvado, SP, Brazil.
出版信息
Vet Med Int. 2011 Apr 3;2011:304349. doi: 10.4061/2011/304349.
Avian mycoplasmosis causes great economic losses to the poultry industry, and one of the major agents involved is Mycoplasma synovie (MS). Serum from commercial poultry breeders (n = 2781) was tested for MS by serum plate agglutination (SPA), hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA). From 2,781 samples tested, 736 (26.46%) were positive in SPA. From 712 SPA-positive sera, 30 samples (4.21%) were positive in HI, and 150 samples (21.06%) were positive in ELISA. Copositivity between ELISA and HI was 90%, and conegativity was 82.0%. Agreement between HI and ELISA was rejected by McNemar's test (P ≤ .001), and Kappa coefficient showed a weak correlation between the two techniques (k = 0.25; 0.21 ≤ k < 0.40). Weak statistical correlation was observed between all serological tests (SPA, HI, and ELISA), and they should only be used for initial screening for MS.
禽支原体病给家禽业造成了巨大的经济损失，其中一个主要致病原是滑膜支原体（MS）。采用血清平板凝集试验（SPA）、血凝抑制试验（HI）和酶联免疫吸附测定（ELISA）对商业家禽养殖者的血清（n = 2781）进行MS检测。在检测的2781份样本中，736份（26.46%）SPA检测呈阳性。在712份SPA阳性血清中，30份（4.21%）HI检测呈阳性，150份（21.06%）ELISA检测呈阳性。ELISA和HI的共同阳性率为90%，共同阴性率为82.0%。McNemar检验拒绝了HI和ELISA之间的一致性（P≤0.001），Kappa系数显示这两种技术之间存在弱相关性（k = 0.25；0.21≤k<0.40）。在所有血清学检测（SPA、HI和ELISA）之间观察到弱的统计学相关性，它们仅应用于MS的初步筛查。
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