研究人类气道上皮细胞分化与修复的方法。
Approaches to study differentiation and repair of human airway epithelial cells.
作者信息
Crespin Sophie, Bacchetta Marc, Huang Song, Dudez Tecla, Wiszniewski Ludovic, Chanson Marc
机构信息
Laboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research, 64 Avenue de la Roseraie, 1211, Geneva, Switzerland.
出版信息
Methods Mol Biol. 2011;742:173-85. doi: 10.1007/978-1-61779-120-8_10.
One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air-surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air-liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.
气道黏膜的主要功能之一是在气-液界面维持机械屏障，并保护呼吸道免受外部损伤。通过在气液界面的微孔膜上培养细胞，可在体外重现人气道上皮细胞（hAECs）向极化气道黏膜的分化。在此，我们描述了使用一种名为MucilAir™的商用气道细胞培养模型来研究hAECs分化及修复的方法。
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