Jiang Di, Schaefer Niccolette, Chu Hong Wei
Department of Medicine, National Jewish Health, Denver, CO, USA.
Methods Mol Biol. 2018;1809:91-109. doi: 10.1007/978-1-4939-8570-8_8.
Air-liquid interface culture enables airway epithelial cells to differentiate into a pseudostratified cell layer, consisting of ciliated cells, goblet/secretory cells, and basal cells (Ghio et al., Part Fibre Toxicol 10:25, 2013). This technique is critically important for in vitro studies of lung diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis, since differentiated airway epithelial cells are more representative of the in vivo lung environment than non-differentiated cells (Derichs et al., FASEB J 25:2325-2332, 2011; Hackett et al., Am J Respir Cell Mol Biol 45:1090-1100, 2011;Schneider et al., Am J Respir Crit Care Med 182: 332-340, 2010). Here we describe the process of isolating and expanding human and mouse airway epithelial cells, as well as differentiation of airway epithelial cells by air-liquid interface culture.
气液界面培养可使气道上皮细胞分化为假复层细胞层，该细胞层由纤毛细胞、杯状/分泌细胞和基底细胞组成（吉奥等人，《部分纤维毒理学》第10卷：第25页，2013年）。这项技术对于哮喘、慢性阻塞性肺疾病和囊性纤维化等肺部疾病的体外研究至关重要，因为与未分化细胞相比，分化的气道上皮细胞更能代表体内肺环境（德里克斯等人，《美国实验生物学会联合会杂志》第25卷：第2325 - 2332页，2011年；哈克特等人，《美国呼吸细胞与分子生物学杂志》第45卷：第1090 - 1100页，2011年；施耐德等人，《美国呼吸与危重症医学杂志》第182卷：第332 - 340页，2010年）。在此，我们描述分离和扩增人和小鼠气道上皮细胞的过程，以及通过气液界面培养使气道上皮细胞分化的过程。
