高效液相色谱-荧光检测法测定中药材及相关制品中的玉米赤霉烯酮

Determination of zearalenone in traditional Chinese medicinal plants and related products by HPLC-FLD.

作者信息

Zhang Xiaofei, Liu Wenkun, Logrieco Antonio F, Yang Meihua, Ou-yang Zhen, Wang Xiong, Guo Qi

机构信息

a Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College , Beijing 100193 , China.

出版信息

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011;28(7):885-93. doi: 10.1080/19440049.2011.563429. Epub 2011 May 5.

DOI:10.1080/19440049.2011.563429

PMID:21547799

Abstract

A HPLC-FLD method has been developed and validated for zearalenone (ZON) in 107 widely consumed Chinese medicinal herbs and related products collected from different regions of China. Samples were extracted with methanol/water (80 : 20, v/v), and the extracts were cleaned-up through immunoaffinity columns (IAC). ZON was quantified by HPLC with fluorescence detection. Recoveries from three different medicinal herbs spiked with ZON at levels ranging from 30 to 600 µg kg(-1) were from 80.8 to 98.3%. The limit of detection was 9.5 µg kg(-1), based on a signal-to-noise ratio of 3 : 1. Naturally occurring ZON was only found in coix seed medicinal herb (all nine samples), with levels ranging from 18.7 to 211.4 µg kg(-1). Positive results were confirmed by UV spectrum and LC-ESI-MS/MS. This is the first report of ZON contamination of a Chinese medicinal herb.

摘要

已建立并验证了一种高效液相色谱 - 荧光检测法（HPLC - FLD），用于检测从中国不同地区收集的107种广泛食用的中药材及相关产品中的玉米赤霉烯酮（ZON）。样品用甲醇/水（80∶20，v/v）提取，提取物通过免疫亲和柱（IAC）净化。ZON通过带荧光检测的HPLC进行定量。在三种不同中药材中添加浓度范围为30至600 μg kg⁻¹的ZON，回收率为80.8%至98.3%。基于3∶1的信噪比，检测限为9.5 μg kg⁻¹。仅在薏苡仁中药材（所有九个样品）中发现天然存在的ZON，含量范围为18.7至211.4 μg kg⁻¹。通过紫外光谱和液相色谱 - 电喷雾串联质谱（LC - ESI - MS/MS）对阳性结果进行了确认。这是关于中药材受ZON污染情况的首次报道。