Glass Research Department, National Research Centre, Dokki, Cairo 12622, Egypt.
J Biomed Mater Res A. 2011 Jul;98(1):132-42. doi: 10.1002/jbm.a.33101. Epub 2011 May 4.
60P₂O₅-20CaO-(20-x) Na₂O-xAg₂O and 60P₂O₅-30CaO-(10-x) Na₂O-xAg₂O glasses, x = 0, 0.5,1, and 2 mol % were prepared using normal glass melting technique. The antibacterial activity of pressed disks of powdered glass (undoped and silver-doped glass) was investigated against S.aureus, P.aeruginosa, and E.coli micro-organisms using agar disk-diffusion assays at 37 °C for 24 h. The antibacterial activity was deduced from the inhibition zone diameter (IZD), zone of no bacterial growth, measured under the stated experimental conditions. The antibacterial activity increases with the increase in IZD and vice versa. Dissolution of glass in water at 37 °C, pH changes of water during glass dissolution, and concentrations of silver ions released from silver-doped glasses into water during their dissolution were determined. An increase in the concentration of silver ions released from silver-doped glasses into water was observed with increasing time of glass dissolution and with increasing Ag₂O content. The tested silver-free and silver-doped glasses demonstrated different antibacterial activity against the tested micro-organisms. For silver-free glasses, an increase in IZD was observed with the increase in the glass dissolution rate and with the decrease in pH of water. Also, the IZD showed an increase with increasing Ag₂O content of silver-doped glasses.
采用常规玻璃熔融技术制备了 60P₂O₅-20CaO-(20-x) Na₂O-xAg₂O 和 60P₂O₅-30CaO-(10-x) Na₂O-xAg₂O 玻璃，其中 x = 0、0.5、1 和 2 mol%。通过琼脂平板扩散法在 37°C 下培养 24 小时，研究了粉末状玻璃（未掺杂和掺杂银的玻璃）压片对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌微生物的抗菌活性。抗菌活性是根据抑菌圈直径（IZD）推断的，即在规定的实验条件下测量的无细菌生长区。抗菌活性随 IZD 的增加而增加，反之亦然。测定了玻璃在 37°C 下的水中溶解、玻璃溶解过程中水中 pH 值的变化以及溶解过程中银掺杂玻璃释放到水中的银离子浓度。随着玻璃溶解时间的增加和 Ag₂O 含量的增加，观察到从掺杂银的玻璃释放到水中的银离子浓度增加。测试的无银和掺银玻璃对测试的微生物表现出不同的抗菌活性。对于无银玻璃，随着玻璃溶解速率的增加和水的 pH 值的降低，IZD 增加。此外，IZD 随着掺银玻璃中 Ag₂O 含量的增加而增加。
