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使用银掺杂硼酸盐生物玻璃去除污水中的大肠杆菌O157:H7大肠菌群细菌。

Removal of E. coli O157:H7 coliform bacteria from sewage wastewater using silver doped borate bioglass.

作者信息

Elsaba Bassant I, Elsayad Ashraf, Rammah Yasser S, Salaheldin Hosam

机构信息

Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt.

Department of Physics, Faculty of Science, Menoufia University, Shebin El-Koom, Menoufia, 32511, Egypt.

出版信息

Sci Rep. 2025 Jul 26;15(1):27212. doi: 10.1038/s41598-025-11844-8.

DOI:10.1038/s41598-025-11844-8
PMID:40715261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12297699/
Abstract

The depletion of freshwater resources constitutes a significant global issue, prompting the consideration of treated sewage wastewater (SWW) reuse as a viable alternative water source within contemporary water management strategies. The current study represents an innovative silver borate-based bioglass (BG) system for the treatment of municipal SWW. The melting-quenching method was used to prepare silver doped BG samples with different AgO concentrations. BAg1, BAg2, BAg3, and BAg4 BG samples were obtained from (65-X) BO-20NaO-10CaO-5PO-XAgO (where X = 0, 1, 2, 3, and 4 mol%). A major fecal coliform bacterium, Escherichia coli O157:H7 (E. coli O157:H7), was used as an indication of SWW purification. BG samples were characterized using FTIR, XRD, SEM, EDX, and DLS, respectively. BAg1 BG sample showed the strongest antibacterial activity against E. coli O157:H7, with a 27.0 ± 1.0 mm inhibition zone and 95.31% growth inhibition. Compared to other BG samples, the inhibition zone diameter was marginally lower than the amoxicillin positive control (28.33 ± 0.1 mm). Additionally, all BG samples inhibit the coliform bacterial community approximately 100%., whereas the original BG sample (BAg0) did not inhibit coliform bacteria. All BG samples had minimum inhibition concentrations (MICs) of 5 mg/mL and minimum bactericidal concentrations (MBCs) of 50 mg/mL, respectively, compared to the untreated bacterial culture (- Ve control). SEM micrographs showed that BAg1 sample treated E. coli O157:H7 cells deteriorated more than BAg0. In conclusion, silver-doped BG is a cost-effective, eco-friendly, and highly recommended technology for SWW purification from fecal coliform bacteria and therefore, can be used for reclamation and other non-drinking water.

摘要

淡水资源的枯竭是一个重大的全球性问题,促使人们在当代水资源管理策略中考虑将处理后的污水回用作为一种可行的替代水源。当前的研究提出了一种创新的基于硼酸银的生物玻璃(BG)系统用于处理城市污水。采用熔融淬火法制备了不同AgO浓度的银掺杂BG样品。BAg1、BAg2、BAg3和BAg4 BG样品由(65-X)BO-20NaO-10CaO-5PO-XAgO(其中X = 0、1、2、3和4 mol%)制得。一种主要的粪便大肠菌,即大肠杆菌O157:H7,被用作污水净化的指示菌。分别使用傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)、扫描电子显微镜(SEM)、能谱仪(EDX)和动态光散射(DLS)对BG样品进行了表征。BAg1 BG样品对大肠杆菌O157:H7表现出最强的抗菌活性,抑菌圈直径为27.0±1.0毫米,生长抑制率为95.31%。与其他BG样品相比,抑菌圈直径略低于阿莫西林阳性对照(28.33±0.1毫米)。此外,所有BG样品对大肠菌群的抑制率约为100%,而原始BG样品(BAg0)对大肠菌没有抑制作用。与未处理的细菌培养物(阴性对照)相比,所有BG样品的最低抑菌浓度(MIC)均为5毫克/毫升,最低杀菌浓度(MBC)均为50毫克/毫升。扫描电子显微镜图像显示,BAg1样品处理后的大肠杆菌O157:H7细胞比BAg0处理后的细胞降解更严重。总之,银掺杂BG是一种经济高效、生态友好且强烈推荐用于从粪便大肠菌中净化污水的技术,因此可用于水的回收利用及其他非饮用水用途。

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