Sleem M M, Eid El-Sayed Gad, Abdelghany A M, Farahat D S
Department of Dental Biomaterials, Faculty of Dentistry, Mansoura University, El-Gomhouria street, Mansoura, 35516, Egypt.
Spectroscopy Department, Physics Research Institute, National Research Centre, 33 Elbehouth street, Dokki, Giza, 12311, Egypt.
BMC Oral Health. 2025 Jul 31;25(1):1284. doi: 10.1186/s12903-025-06665-0.
White spot lesions represent the first clinical sign of dental caries and can be reversed using various remineralizing agents. This study aimed to synthesize different bioactive glass varnishes and assess their remineralizing effects on white spot lesions compared to fluoride varnish and a synthesized nanosilver fluoride varnish.
Seventy-two extracted human teeth were used. The teeth were divided into seven groups (n = 10): Group A (Artificial saliva, negative control), Group B (Fluoride varnish), Group C (Bioactive glass varnish), Group D (Fluoride-containing bioactive glass varnish), Group E (Nanosilver-containing bioactive glass varnish), Group F (Nanosilver fluoride varnish), and Group G (Nanosilver varnish). The different varnishes were synthesized and then characterized via transmission electron microscopy and UV-vis spectroscopy. Artificial caries were induced in the specimens by immersion in a demineralizing solution for 4 days. Each specimen was analyzed at baseline and in the demineralized state and after two weeks of varnish application by Energy Dispersive X-ray analysis and Vickers micro-hardness tester. One representative specimen from each group, one specimen at baseline, and one demineralized specimen were examined using scanning electron microscopy. Statistical analysis was conducted using one-way analysis of variance (ANOVA) and repeated measures ANOVA followed by Tukey's post hoc test.
The nanosilver-containing bioactive glass group (E) presented the highest mineral gain percentage, followed by fluoride-containing bioactive glass (D) and nanosilver fluoride (F) groups, with insignificant differences between groups D and F. Groups D, E, and F showed the highest hardness recovery percentages, with insignificant differences among them. Scanning electron microscopy revealed the development of new crystals in these groups.
Bioactive glass varnish is a promising remineralizing agent with efficacy comparable to fluoride varnish. The fluoride-containing and nanosilver-containing bioactive glass varnishes had a higher remineralizing potential compared to the fluoride and bioactive glass varnishes and nearly the same remineralizing effect of the nanosilver fluoride varnish.
白斑病变是龋齿的首个临床症状，可使用多种再矿化剂使其逆转。本研究旨在合成不同的生物活性玻璃清漆，并与氟化物清漆和合成的纳米银氟化物清漆相比，评估它们对白斑病变的再矿化效果。
使用72颗拔除的人牙。将牙齿分为七组（n = 10）：A组（人工唾液，阴性对照）、B组（氟化物清漆）、C组（生物活性玻璃清漆）、D组（含氟生物活性玻璃清漆）、E组（含纳米银生物活性玻璃清漆）、F组（纳米银氟化物清漆）和G组（纳米银清漆）。合成不同的清漆，然后通过透射电子显微镜和紫外可见光谱对其进行表征。通过将标本浸入脱矿溶液中4天来诱导人工龋齿。在基线、脱矿状态以及涂抹清漆两周后，使用能量色散X射线分析和维氏显微硬度测试仪对每个标本进行分析。使用扫描电子显微镜检查每组的一个代表性标本、一个基线标本和一个脱矿标本。使用单因素方差分析（ANOVA）和重复测量ANOVA，随后进行Tukey事后检验进行统计分析。
含纳米银生物活性玻璃组（E）的矿物质增加百分比最高，其次是含氟生物活性玻璃（D）组和纳米银氟化物（F）组，D组和F组之间差异不显著。D组、E组和F组的硬度恢复百分比最高，它们之间差异不显著。扫描电子显微镜显示这些组中有新晶体形成。
生物活性玻璃清漆是一种有前景的再矿化剂，其功效与氟化物清漆相当。与氟化物清漆和生物活性玻璃清漆相比，含氟和含纳米银的生物活性玻璃清漆具有更高的再矿化潜力，且与纳米银氟化物清漆的再矿化效果几乎相同。
