Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
Proteomics. 2011 Jun;11(11):2294-307. doi: 10.1002/pmic.200900690. Epub 2011 May 5.
The goal of this study was to identify promising new biomarkers of cadmium by identifying differentially expressed proteins in Paronychiurus kimi after exposure to cadmium. Through proteomic analysis of P. kimi using 1-D PAGE and nano-LC-MS/MS, 36 downregulated proteins and 40 upregulated proteins were found. Some of the downregulated and upregulated proteins were verified by LC-MS/MS analysis after 2-D PAGE. Downregulated proteins in response to cadmium exposure were involved in glycolysis and energy metabolism, chaperones, transcription, reproduction, and neuron growth. In contrast, proteins involved in glycolysis and energy production, neurogenesis, defense systems response to bacteria, and protein biosynthesis were upregulated in cadmium-treated collembolans. Cubulin may be a potential biomarker for the detection of cadmium in P. kimi since this biomarker was able to low levels (3.5 mg/kg) of cadmium. The 14-3-3 ζ was also found to be a potential biomarker for the detection of medium levels (14 mg/kg) of cadmium. Collembolans may be an alternative tool to humans because many collembolans proteins show a high homology to human proteins.
本研究的目的是通过鉴定接触镉后 Paronychiurus kimi 中差异表达的蛋白质，来确定有前途的新镉生物标志物。通过对 P. kimi 进行 1-D PAGE 和纳升 LC-MS/MS 的蛋白质组学分析，发现了 36 个下调蛋白和 40 个上调蛋白。一些下调和上调蛋白在经过 2-D PAGE 后的 LC-MS/MS 分析中得到了验证。对镉暴露的响应而下调的蛋白参与糖酵解和能量代谢、伴侣蛋白、转录、生殖和神经元生长。相比之下，在镉处理的弹尾目动物中，参与糖酵解和能量产生、神经发生、细菌防御系统反应以及蛋白质生物合成的蛋白上调。由于该生物标志物能够检测到低水平（3.5mg/kg）的镉，因此 Cubulin 可能是检测 P. kimi 中镉的潜在生物标志物。还发现 14-3-3 ζ 也是检测中水平（14mg/kg）镉的潜在生物标志物。由于许多弹尾目动物蛋白与人蛋白具有高度同源性，因此弹尾目动物可能是人类的替代工具。
