Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
Proteomics. 2011 Aug;11(15):3086-105. doi: 10.1002/pmic.201000818. Epub 2011 May 5.
Diverse bacterial taxa that live in association with plants affect plant health and development. This is most evident for those bacteria that undergo a symbiotic association with plants or infect the plants as pathogens. Proteome analyses have contributed significantly toward a deeper understanding of the molecular mechanisms underlying the development of these associations. They were applied to obtain a general overview of the protein composition of these bacteria, but more so to study effects of plant signaling molecules on the cytosolic proteome composition or metabolic adaptations upon plant colonization. Proteomic analyses are particularly useful for the identification of secreted proteins, which are indispensable to manipulate a host plant. Recent advances in the field of proteome analyses have initiated a new research area, the analysis of more complex microbial communities. Such studies are just at their beginning but hold great potential for the future to elucidate not only the interactions between bacteria and their host plants, but also of bacteria-bacteria interactions between different bacterial taxa when living in association with plants. These include not only the symbiotic and pathogenic bacteria, but also the commensal bacteria that are consistently found in association with plants and whose functions remain currently largely uncovered.
与植物共生的各种细菌类群会影响植物的健康和发育。对于那些与植物形成共生关系或作为病原体感染植物的细菌来说，这一点尤为明显。蛋白质组分析为深入了解这些关联的发展所涉及的分子机制做出了重大贡献。它们不仅用于获得这些细菌蛋白质组成的总体概述，而且更用于研究植物信号分子对细胞质蛋白质组组成的影响，或植物定植后的代谢适应。蛋白质组分析对于鉴定分泌蛋白特别有用，这些蛋白对于操纵宿主植物是不可或缺的。蛋白质组分析领域的最新进展开创了一个新的研究领域，即更复杂微生物群落的分析。这些研究才刚刚开始，但具有很大的潜力，可以阐明不仅是细菌与其宿主植物之间的相互作用，而且还有当不同细菌类群与植物共生时，细菌之间的相互作用。这些细菌不仅包括共生和病原菌，还包括与植物持续共生但目前其功能仍很大程度上未被发现的共生菌。
