Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310032, PR China.
N Biotechnol. 2011 Oct;28(6):610-5. doi: 10.1016/j.nbt.2011.04.004. Epub 2011 Apr 27.
2-Chloronicotinic acid is receiving much attention for its effective applications as a key precursor in the synthesis of pesticides and medicines. In this study, a strain ZJB-09149 converting 2-chloro-3-cyanopyridine to 2-chloronicotinic acid was newly isolated and identified as Rhodococcus erythropolis, based on its physiological and biological tests, and 16S rDNA sequence analysis. In addition, the effects of inducer, carbon source and nitrogen source were examined. Maximum activity was achieved when the above parameters were set as 8 g/l ɛ-caprolactam, 7 g/l yeast extract and 5 g/l maltose. Moreover, the biotransformation pathway of 2-chloro-3-cyanopyridine to 2-chloronicotinic acid in strain ZJB-09149 was investigated as well. This study revealed that the nitrile hydratase (NHase) and amidase expressed in R. erythropolis ZJB-09149 are responsible for the conversion of 2-chloro-3-cyanopyridine. This is the first time to report on the biotransformation preparation of 2-chloronicotinic acid.
2-氯烟酸作为农药和医药合成的关键前体，其有效应用受到广泛关注。本研究通过生理生化试验和 16S rDNA 序列分析，从土壤中分离得到一株能将 2-氯-3-氰基吡啶转化为 2-氯烟酸的菌株 ZJB-09149，并鉴定为红球菌（Rhodococcus erythropolis）。此外，还考察了诱导剂、碳源和氮源对酶转化的影响。当ɛ-己内酰胺、酵母提取物和麦芽糖的浓度分别为 8 g/L、7 g/L 和 5 g/L 时，菌株 ZJB-09149 的酶活最高。进一步研究了菌株 ZJB-09149 转化 2-氯-3-氰基吡啶生成 2-氯烟酸的生物转化途径，结果表明红球菌 ZJB-09149 中表达的腈水合酶（NHase）和酰胺酶负责 2-氯-3-氰基吡啶的转化。这是首次报道利用生物转化法制备 2-氯烟酸。
