Chatham W W, Heck L W, Blackburn W D
Division of Clinical Immunology and Rheumatology, Birmingham Veterans Administration Hospital, AL 35233.
Arthritis Rheum. 1990 Feb;33(2):228-34. doi: 10.1002/art.1780330211.
The amount of active neutrophil (PMN) collagenase released extracellularly is dependent on the PMN-activating ligand. Neutrophils stimulated with soluble ligands, including FMLP, platelet-activating factor, or heat-aggregated IgG, released very little active collagenase, in contrast to cells stimulated with opsonized zymosan or surface-bound IgG. However, opsonized zymosan and surface-bound IgG did not differ appreciably from soluble ligands in effecting PMN production of superoxide, release of the specific granule component lactoferrin, or total (latent plus active) collagenase release, which suggests that there is more efficient collagenase activation during PMN stimulation with surface-bound ligands. These results suggest a role for surface (cartilage)-bound IgG in the release and activation of human neutrophil collagenase in the joints of patients with rheumatoid arthritis.
细胞外释放的活性中性粒细胞（PMN）胶原酶的量取决于PMN激活配体。与用调理酵母聚糖或表面结合的IgG刺激的细胞相比，用可溶性配体（包括FMLP、血小板活化因子或热聚集IgG）刺激的中性粒细胞释放的活性胶原酶非常少。然而，调理酵母聚糖和表面结合的IgG在影响PMN产生超氧化物、释放特异性颗粒成分乳铁蛋白或总（潜伏加活性）胶原酶释放方面与可溶性配体没有明显差异，这表明在用表面结合配体刺激PMN期间存在更有效的胶原酶激活。这些结果提示表面（软骨）结合的IgG在类风湿性关节炎患者关节中人类中性粒细胞胶原酶的释放和激活中起作用。
