末端结合蛋白 EB3 和 EB1 将微管与轴突起始段的锚蛋白 G 连接起来。
End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment.
机构信息
Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche 641, F-13916 Marseille, France.
出版信息
Proc Natl Acad Sci U S A. 2011 May 24;108(21):8826-31. doi: 10.1073/pnas.1018671108. Epub 2011 May 6.
Abstract
The axon initial segment (AIS) plays a key role in maintaining the molecular and functional polarity of the neuron. The relationship between the AIS architecture and the microtubules (MTs) supporting axonal transport is unknown. Here we provide evidence that the MT plus-end-binding (EB) proteins EB1 and EB3 have a role in the AIS in addition to their MT plus-end tracking protein behavior in other neuronal compartments. In mature neurons, EB3 is concentrated and stabilized in the AIS. We identified a direct interaction between EB3/EB1 and the AIS scaffold protein ankyrin G (ankG). In addition, EB3 and EB1 participate in AIS maintenance, and AIS disassembly through ankG knockdown leads to cell-wide up-regulation of EB3 and EB1 comets. Thus, EB3 and EB1 coordinate a molecular and functional interplay between ankG and the AIS MTs that supports the central role of ankG in the maintenance of neuronal polarity.
摘要
轴突起始段（AIS）在维持神经元的分子和功能极性方面起着关键作用。AIS 结构与支持轴突运输的微管（MT）之间的关系尚不清楚。在这里，我们提供的证据表明，除了在其他神经元区室中的 MT 末端结合（EB）蛋白 EB1 和 EB3 的 MT 末端追踪蛋白行为外，它们在 AIS 中也具有作用。在成熟的神经元中，EB3 集中并稳定在 AIS 中。我们确定了 EB3/EB1 与 AIS 支架蛋白锚蛋白 G（ankG）之间的直接相互作用。此外，EB3 和 EB1 参与 AIS 的维持，通过 ankG 敲低导致 AIS 解体，从而导致 EB3 和 EB1 彗星在全细胞范围内上调。因此，EB3 和 EB1 协调 ankG 与 AIS MT 之间的分子和功能相互作用，支持 ankG 在维持神经元极性中的核心作用。
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