Department of Anatomy, University of Göteborg, Göteborg (Sweden).
Restor Neurol Neurosci. 1990 Jan 1;1(3):253-9. doi: 10.3233/RNN-1990-13412.
The silicone nerve regeneration chamber is a useful model to investigate the cellular and molecular events underlying successful regeneration in the peripheral nervous system. In this model a transected rat sciatic nerve with a 10-mm interstump gap, is repaired with a silicone chamber. The spatial-temporal sequence of regeneration in the silicone chamber has been examined in detail. The chamber rapidly becomes filled with fluid which contains neurotrophic activity for neurons in vitro. The second event to occur is the formation of a fibrin matrix connecting the two nerve stumps. This matrix is then invaded by cellular elements in the following order: perineurial-like cells, vasculature, Schwann cells, and axons. The silicone chamber model also allows manipulation of the regeneration process. Prefilling the chamber at the time of implantation with phosphate-buffered saline or dialyzed plasma stimulates nerve regeneration. Multiple injections into the chamber of a mixture containing laminin, testosterone, and ganglioside GM1 increase the size and the vascularization of the regenerate. Specially designed chambers divided into two compartments by a longitudinally inserted nitrocellulose strip have been used to examine the effects of substrate-bound trophic factors on nerve regeneration. Fibroblast growth factor containing chambers have an improved regeneration and vascularization as compared to controls.
硅橡胶神经再生室是研究周围神经系统成功再生的细胞和分子事件的有用模型。在该模型中，大鼠坐骨神经被横断，两个断端之间有 10mm 的神经缺损，用硅橡胶管修复。硅橡胶管内的再生过程的时空顺序已经被详细研究。管内迅速充满液体，其中含有体外神经元的神经营养活性。接下来发生的第二个事件是形成连接两个神经断端的纤维蛋白基质。然后，以下顺序的细胞成分侵入该基质：类雪旺细胞、脉管系统、施万细胞和轴突。硅橡胶管模型还允许对再生过程进行操作。在植入时预先用磷酸盐缓冲盐水或透析血浆填充管腔，可以刺激神经再生。向管腔中多次注射含有层粘连蛋白、睾丸酮和神经节苷脂 GM1 的混合物，可以增加再生的大小和血管化。通过在纵向插入的硝酸纤维素条将特制的管腔分成两个隔室，用于研究基底结合的营养因子对神经再生的影响。含有成纤维细胞生长因子的管腔与对照组相比，具有改善的再生和血管化。
