Departments of Experimental Research and Hand Surgery, Malmö General Hospital, Lund University, Malmö, Sweden.
Restor Neurol Neurosci. 1994 Jan 1;6(4):317-22. doi: 10.3233/RNN-1994-6408.
The silicone chamber model for nerve regeneration is suitable to test the effects of exogenous agents or surgical manipulations on nerve regeneration. The total 16-day regeneration period used in this model makes it possible to analyze the effects of certain manipulations on the sequential advancement of the individual cellular components (circumferential perineurial-like cells, vessels, Schwann cells, axons, and myelin) into the chamber fibrin matrix. In the present study we compared the effects on cellular migration of a 7 day delayed chamber repair vs. chamber repair immediately after transection (control chambers) of the rat sciatic nerve. Regeneration was evaluated with light and electron microscopic techniques. Chambers implanted after a delay of 7 days had a statistically significant more advanced migration of vessels, Schwann cells, and axons from the proximal nerve stump and also a significantly increased vascular density as compared to control chambers. We conclude that a 7 day delayed nerve repair stimulates nerve regeneration in this specific silicone chamber model.
硅橡胶室模型适合于测试外源物质或外科操作对神经再生的影响。该模型的 16 天总再生期使得分析某些操作对单个细胞成分（环形类神经外膜细胞、血管、施万细胞、轴突和髓鞘）进入室纤维蛋白基质的顺序进展的影响成为可能。在本研究中，我们比较了在大鼠坐骨神经切断后 7 天延迟室修复与即刻室修复（对照室）对细胞迁移的影响。通过光镜和电镜技术对再生进行评估。与对照室相比，延迟 7 天植入的室具有统计学上更显著的来自近端神经残端的血管、施万细胞和轴突的更先进的迁移，并且血管密度也显著增加。我们得出结论，7 天延迟的神经修复刺激了该特定硅橡胶室模型中的神经再生。
