Williams L R, Danielsen N, Müller H, Varon S
Department of Neurosciences, School of Medicine, University of California San Diego, La Jolla 92093.
J Comp Neurol. 1987 Oct 8;264(2):284-90. doi: 10.1002/cne.902640211.
When silicone regeneration chambers are implanted empty, axonal regeneration fails if the interstump gap length is greater than 10 mm. Previous experiments using the 10-mm gap model demonstrated that regeneration success correlated with the dimension and/or consistency of the naturally formed acellular fibrin matrix. Both spatial and temporal parameters of regeneration could be stimulated through modifications of the fibrin matrix by prefilling the chambers at the time of implantation either with phosphate-buffered saline or plasma dialyzed against phosphate-buffered saline. In the present experiments, similar modification of matrix formation was found to promote successful regeneration across 15-mm and 20-mm interstump gap lengths. In addition, prefilling 15-mm-gap chambers with dialyzed plasma resulted in a 3.5-fold increase in the incidence of functional restitution detected at 8 weeks after implantation over the outcome with chambers prefilled with phosphate-buffered saline.
当硅酮再生腔为空植入时，如果残端间隙长度大于10毫米，轴突再生就会失败。先前使用10毫米间隙模型的实验表明，再生成功与天然形成的无细胞纤维蛋白基质的尺寸和/或稠度相关。通过在植入时用磷酸盐缓冲盐水或用磷酸盐缓冲盐水透析的血浆预填充腔室来改变纤维蛋白基质，可以刺激再生的空间和时间参数。在本实验中，发现对基质形成进行类似的改变可促进在15毫米和20毫米残端间隙长度上的成功再生。此外，用透析血浆预填充15毫米间隙腔室，与用磷酸盐缓冲盐水预填充的腔室相比，植入后8周检测到的功能恢复发生率增加了3.5倍。
