Department of Zoophysiology, University of Lund, Lund (Sweden).
Restor Neurol Neurosci. 1990 Jan 1;1(3):261-6. doi: 10.3233/RNN-1990-13413.
The adult frog sciatic nerve offers several advantages as an in vitro model to study nerve regeneration. The nerve with the attached dorsal root ganglia can easily be isolated and incubated in a culture medium for several days. If the nerve is subjected to a crush immediately after dissection there is a delay of 3.4 days after which the sensory axons start to regenerate into the distal nerve stump at a constant rate of about 1.1 mm · day-1 in serum-containing and 1.0 mm · day-1 in serum-free medium. Serum-free cultures may be used in future studies to examine the effect of various neurotrophic factors. The existence of an accurate method for examining the outgrowth distance, based on axonal transport of labelled proteins, contributes to the attractiveness of the model. A compartmental culture system permits separate exposure of the ganglia and the nerve to different agents. Taking advantage of this, pharmacological studies suggest that Schwann cells produce signals, dependent on newly transcribed RNA, which transform the preparation into a growth state. The present model system offers favourable conditions to learn more about the early events and also the subsequent steps of the regeneration process.
成年青蛙坐骨神经作为一种体外模型，具有许多优点，可用于研究神经再生。带有背根神经节的神经很容易被分离出来，并在培养基中培养数天。如果在解剖后立即对神经进行挤压，那么在血清培养物中，感觉轴突在 3.4 天后开始以约 1.1mm·天-1的恒定速率再生到远端神经残端，而在无血清培养基中则以 1.0mm·天-1的速率再生。无血清培养物可用于未来的研究，以检查各种神经营养因子的影响。该模型的吸引力在于存在一种基于标记蛋白轴突运输的精确方法来检查生长距离。分隔培养系统允许将神经节和神经分别暴露于不同的试剂。利用这一点，药理学研究表明施万细胞产生依赖于新转录 RNA 的信号，将该制剂转变为生长状态。目前的模型系统为深入了解再生过程的早期事件和随后的步骤提供了有利的条件。
