General Infirmary, Leeds (U.K.) and University of Leeds, Leeds (U.K.).
Restor Neurol Neurosci. 1991 Jan 1;2(4):251-4. doi: 10.3233/RNN-1991-245613.
Portions of 1 cm length of the sensory radial nerve from the cat forelimb were used to replace an excised portion of the dorsal columns in the upper lumbar spinal cord. Observations were made on the clinical recovery of the animals, and cine recordings were made of their ability to traverse a horizontal ladder 5 months after the grafting procedure. Evoked sensory potential studies performed 6 months after grafting showed that an impulse arising from a stimulus applied to the sciatic nerve could be recorded in the spinal cord caudal to the graft, in the graft and in the spinal cord rostral to the graft in 5 out of 8 animals. Tracing of nerve connections with injection of horseradish peroxidase into the grafts resulted in labelling of nerve cell bodies in dorsal root ganglia and the grey matter of the lumbar spinal cord up to a distance of 10 mm away from the graft. These results confirm that peripheral nerve grafts can provide a satisfactory environment for the regrowth of ascending fibres in the dorsal columns of the spinal cord. However, there is as yet no evidence that the regenerated fibres succeed in forming useful synaptic connections with other nerve cell bodies.
从猫的前肢中取出 1 厘米长的感觉桡神经的一部分，用于替代上腰椎脊髓中切除的一部分背柱。观察动物的临床恢复情况，并在移植后 5 个月对它们穿越水平梯的能力进行电影记录。移植后 6 个月进行的诱发感觉电位研究表明，在 8 只动物中的 5 只中，从坐骨神经施加刺激产生的冲动可以在移植后的脊髓、移植和移植前的脊髓中记录到。将辣根过氧化物酶注入移植物中追踪神经连接，导致背根神经节和腰椎脊髓灰质中的神经细胞体标记，距离移植物 10 毫米远。这些结果证实，外周神经移植物可以为脊髓背柱中上行纤维的再生提供一个满意的环境。然而，目前还没有证据表明再生纤维成功地与其他神经细胞体形成有用的突触连接。
