Wardrope J, Wilson D H
Paraplegia. 1986 Nov-Dec;24(6):370-8. doi: 10.1038/sc.1986.55.
Excision of the dorsal columns was used to create a lesion in the spinal cord. The defect was bridged using a peripheral nerve graft. Silver staining showed the grafts being invaded by axons. Neuronal tracing by injecting horseradish peroxidase into the graft showed the origin of these fibres to be in dorsal root ganglia and in the grey matter of the lumbar cord. All of the animals studied showed horseradish peroxidase staining of neurones in the grey matter of the lumbar cord caudal to the graft. Five of the animals had staining of the dorsal root ganglion cells. Electro-physiological assessment was performed 5 to 6 months following the grafting procedure. Evoked potentials were recorded from the graft in six animals and from the dorsal columns above the graft in six animals.
切除背柱以在脊髓中制造损伤。使用周围神经移植物桥接缺损。银染色显示移植物被轴突侵入。通过向移植物中注射辣根过氧化物酶进行神经元追踪，结果显示这些纤维起源于背根神经节和腰髓灰质。所有研究的动物在移植物尾侧的腰髓灰质中均显示辣根过氧化物酶对神经元的染色。五只动物的背根神经节细胞有染色。在移植手术后5至6个月进行电生理评估。在六只动物的移植物中以及在六只动物移植物上方的背柱中记录诱发电位。
