Cincinnati Veterans Affairs Medical Center and Departments of Neurology and Physiology/Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH 45220 (U.S.A.).
Restor Neurol Neurosci. 1991 Jan 1;2(4):309-25. doi: 10.3233/RNN-1991-245621.
This review summarizes a series of experiments involving transplants of embryonic feline CNS tissue into chronic compression lesions of the adult cat spinal cord. Fetal spinal cord (FSC), caudal brainstem (BSt), neocortex (NCx) or a combination of either FSC/NCx or FSC/BSt was transplanted as solid pieces or as a suspension of dissociated cells into the developed cystic cavities produced by static-load compression trauma 2-10 weeks prior to grafting. All cats were immunosuppressed with cyclosporin A and their locomotor function was assessed for 6-30 weeks. Following the period of evaluation, all recipients were perfused with fixative and tissue specimens, taken at the transplantation site, were processed for general histological and/or immunocytochemical analysis. Viable graft tissue was found in all animals with the exception of two cats which showed active rejection of their transplants. All of the viable intraspinal grafts were extensively vascularized and did not show any signs of imminent or on-going tissue rejection. Fetal cat CNS grafts showed an extended maturational phase in that features of immature neural tissue (e.g. a paucity of myelination) were still seen even 6-9 weeks after transplantation. By 20-30 weeks, FSC and BSt grafts had attained a more advanced stage of maturation. Transplants in these chronic lesions were extensively blended with both the gray and white matter of the host spinal cord and could be visualized by magnetic resonance imaging (MRI). MRI could also detect regions of cavitation at the graft-host interface, as well as within some transplants. While preliminary evidence from behavioral studies suggest that the FSC and BSt grafts may improve or spare locomotor function in some recipients, a more rigorous analysis of post-grafting locomotor function is required to determine conclusively the functionality of these transplants.
这篇综述总结了一系列实验，这些实验涉及将胚胎猫中枢神经系统组织移植到成年猫脊髓慢性压迫损伤中。胎儿脊髓 (FSC)、尾脑 (BSt)、新皮质 (NCx) 或 FSC/NCx 或 FSC/BSt 的组合作为实体块或分离细胞悬液被移植到在移植前 2-10 周通过静态负荷压迫创伤产生的已发育的囊性腔中。所有猫均接受环孢素 A 免疫抑制治疗，并在 6-30 周内评估其运动功能。在评估期结束后，所有受者均用固定剂灌注，在移植部位采集组织标本，进行一般组织学和/或免疫细胞化学分析。除了两只显示移植物主动排斥的猫外，所有动物均发现有存活的移植物组织。所有存活的脊髓内移植物均广泛血管化，没有任何即将发生或正在进行的组织排斥迹象。胎猫中枢神经系统移植物表现出延长的成熟阶段，即使在移植后 6-9 周，仍可见不成熟神经组织的特征（例如髓鞘形成不足）。到 20-30 周时，FSC 和 BSt 移植物已达到更成熟的阶段。在这些慢性病变中，移植物与宿主脊髓的灰质和白质广泛融合，可通过磁共振成像 (MRI) 检测到。MRI 还可以检测到移植物-宿主界面和某些移植物内的空洞化区域。虽然来自行为研究的初步证据表明，FSC 和 BSt 移植物可能改善或保留一些受者的运动功能，但需要更严格地分析移植后的运动功能，以确定这些移植物的功能。
