Giovanini M A, Reier P J, Eskin T A, Wirth E, Anderson D K
Department of Neurosurgery, University of Florida College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
Exp Neurol. 1997 Dec;148(2):523-43. doi: 10.1006/exnr.1997.6703.
The present study evaluated the growth potential and differentiation of human fetal spinal cord (FSC) tissue in the injured adult rat spinal cord under different lesion and grafting conditions. Donor tissue at 6-9 weeks of gestational age was obtained through elective abortions and transplanted either immediately into acute resection (solid grafts) or into chronic contusion (suspension and solid grafts) lesions (i.e., 14-40 days after injury) in the thoracic spinal cord. The xenografts were then examined either histologically in plastic sections or immunocytochemically 1-3 months postgrafting. Intraspinal grafts in acute lesions demonstrated an 83% survival rate and developed as well-circumscribed nodules that were predominantly composed of immature astrocytes. Solid-piece grafts in chronic contusion lesions exhibited a 92% survival rate and also developed as nodular masses. These grafts, however, contained many immature neurons 2 months postgrafting. Suspension grafts in chronic contusion lesions had an 85% survival rate and expanded in a nonrestrictive, diffuse pattern. These transplants demonstrated large neuronally rich areas of neural parenchyma. Extensive neuritic outgrowth could also be seen extending from these grafts into the surrounding host spinal cord. These findings show that human FSC tissue reliably survives and differentiates in both acute and chronic lesions. However, both the lesion environment and the grafting techniques can greatly influence the pattern of differentiation and degree of host-graft integration achieved.
本研究评估了在不同损伤和移植条件下，人胎儿脊髓（FSC）组织在成年大鼠脊髓损伤部位的生长潜力和分化情况。通过选择性流产获取孕龄6 - 9周的供体组织，并立即将其移植到胸段脊髓的急性切除损伤部位（实体移植）或慢性挫伤损伤部位（悬浮移植和实体移植）（即损伤后14 - 40天）。然后在移植后1 - 3个月，对异种移植组织进行组织学塑料切片检查或免疫细胞化学检查。急性损伤中的脊髓内移植组织存活率为83%，并形成界限清晰的结节，主要由未成熟星形胶质细胞组成。慢性挫伤损伤中的实体移植组织存活率为92%，也形成结节状肿块。然而，这些移植组织在移植后2个月含有许多未成熟神经元。慢性挫伤损伤中的悬浮移植组织存活率为85%，以非限制性、弥漫性模式扩展。这些移植组织显示出富含神经元的大量神经实质区域。从这些移植组织延伸到周围宿主脊髓的广泛神经突生长也可见到。这些发现表明，人FSC组织在急性和慢性损伤中均能可靠地存活并分化。然而，损伤环境和移植技术均可极大地影响分化模式以及宿主与移植组织的整合程度。
