潜伏性爱泼斯坦-巴尔病毒基因组在人上皮细胞/伯基特淋巴母细胞杂交细胞中的表达。
Expression of latent Epstein-Barr virus genomes in human epithelial/Burkitt's lymphoblastoid hybrid cells.
作者信息
Nonoyama M, Tanaka A, Glaser R
出版信息
IARC Sci Publ (1971). 1978(20):397-402.
Expression of latent Epstein-Barr virus genomes in somatic-cell hybrids of Burkitt's lymphoblastoid cells has been studied. Treatment of the hybrid cells, D98/Raji and D98/HR-1, with IUDR induced the formation of EA and VCA and replication of virus DNA, whereas the same treatment of Raji cells induced only the formation of EA. The patterns of transcription of virus genomes in these three cell lines were, however, very similar: 25% without treatment with IUDR, 30% immediately after the treatment and 50% (entire genome transcription) three days after being transferred to fresh medium. The amount of virus RNA in the cells, calculated from DNA-RNA hybridization kinetics, was proportional to the number of virus genomes per cell, suggesting that every copy of virus DNA in these cells is actively transcribed.
对伯基特淋巴母细胞样细胞的体细胞杂种中潜伏性EB病毒基因组的表达进行了研究。用碘苷（IUDR）处理杂种细胞D98/Raji和D98/HR-1，可诱导早期抗原（EA）和病毒衣壳抗原（VCA）的形成以及病毒DNA的复制，而对Raji细胞进行相同处理仅诱导EA的形成。然而，这三种细胞系中病毒基因组的转录模式非常相似：未用IUDR处理时为25%，处理后立即为30%，转移到新鲜培养基三天后为50%（全基因组转录）。根据DNA-RNA杂交动力学计算，细胞中病毒RNA的量与每个细胞中病毒基因组的数量成正比，这表明这些细胞中病毒DNA的每一个拷贝都在活跃转录。
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