Ablashi D V, Glaser R, Easton J M, Nonoyama M, Armstrong G R
Exp Hematol. 1978 Apr;6(4):365-74.
We studied tumor induction in athymic nude mice by D98/HR-1 cells, an epithelial somatic cell hybrid containing the Epstein-Barr virus (EBV) genome, and by the parental D98 and HR-1 cells. Groups of animals were inoculated with cells grown in culture, with cells from tumors induced by the cell lines, or with cells from lines derived from tumors. The tumors induced by D98/HR-1 cells were undifferentiated carcinomas; those induced by D98 cells were carcinomas and those induced by HR-1 cells were poorly differentiated lymphomas. Preliminary data suggest that the number of EBV genome equivalents was sharply reduced in cells from both D98/HR-1 and HR-1 tumors. Subsequent passage of tumor cells in vitro resulted in a partial recovery in the number of EBV genome equivalents in HR-1 cells and a complete recovery in D98/HR-1 cells. The reduction in the number of EBV genomes in the tumor cells suggests that in vitro passage can influence the number of EBV genomes in these cells.
我们研究了含爱泼斯坦-巴尔病毒（EBV）基因组的上皮体细胞杂交瘤D98/HR-1细胞、亲本D98细胞和HR-1细胞在无胸腺裸鼠体内诱发肿瘤的情况。将动物分组，分别接种培养生长的细胞、由这些细胞系诱发肿瘤所产生的细胞，或源自肿瘤的细胞系中的细胞。D98/HR-1细胞诱发的肿瘤为未分化癌；D98细胞诱发的肿瘤为癌，HR-1细胞诱发的肿瘤为低分化淋巴瘤。初步数据表明，D98/HR-1肿瘤细胞和HR-1肿瘤细胞中的EBV基因组当量数均大幅减少。随后肿瘤细胞的体外传代导致HR-1细胞中EBV基因组当量数部分恢复，D98/HR-1细胞中则完全恢复。肿瘤细胞中EBV基因组数量的减少表明体外传代可影响这些细胞中EBV基因组的数量。
