螺内酯对摄入高盐的自发性高血压成年大鼠的影响。
Effects of spironolactone in spontaneously hypertensive adult rats subjected to high salt intake.
机构信息
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES, Brazil.
出版信息
Clinics (Sao Paulo). 2011;66(3):477-82. doi: 10.1590/s1807-59322011000300020.
OBJECTIVE
To evaluate the effect of spironolactone on ventricular stiffness in spontaneously hypertensive adult rats subjected to high salt intake.
INTRODUCTION
High salt intake leads to cardiac hypertrophy, collagen accumulation and diastolic dysfunction. These effects are partially mediated by cardiac activation of the renin-angiotensin-aldosterone system.
METHODS
Male spontaneously hypertensive rats (SHRs, 32 weeks) received drinking water (SHR), a 1% NaCl solution (SHR-Salt), or a 1% NaCl solution with a daily subcutaneous injection of spironolactone (80 mg.kg⁻¹) (SHRSalt- S). Age-matched normotensive Wistar rats were used as a control. Eight weeks later, the animals were anesthetized and catheterized to evaluate left ventricular and arterial blood pressure. After cardiac arrest, a double-lumen catheter was inserted into the left ventricle through the aorta to obtain in situ left ventricular pressure-volume curves.
RESULTS
The blood pressures of all the SHR groups were similar to each other but were different from the normotensive controls (Wistar = 109 ± 2; SHR = 118 ± 2; SHR-Salt = 117 ± 2; SHR-Salt-S = 116 ± 2 mmHg; P < 0.05). The cardiac hypertrophy observed in the SHR was enhanced by salt overload and abated by spironolactone (Wistar = 2.90 ± 0.06; SHR = 3.44 ± 0.07; SHR-Salt = 3.68 ± 0.07; SHR-Salt-S = 3.46 ± 0.05 mg/g; P < 0.05). Myocardial relaxation, as evaluated by left ventricular dP/dt, was impaired by salt overload and improved by spironolactone (Wistar = -3698 ± 92; SHR = -3729 ± 125; SHR-Salt = -3342 ± 80; SHR-Salt-S = -3647 ± 104 mmHg/s; P < 0.05). Ventricular stiffness was not altered by salt overload, but spironolactone treatment reduced the ventricular stiffness to levels observed in the normotensive controls (Wistar = 1.40 ± 0.04; SHR = 1.60 ± 0.05; SHR-Salt = 1.67 ± 0.12; SHR-Salt- S = 1.45 ± 0.03 mmHg/ml; P < 0.05).
CONCLUSION
Spironolactone reduces left ventricular hypertrophy secondary to high salt intake and ventricular stiffness in adult SHRs.
目的
评估螺内酯对摄入高盐的自发性高血压成年大鼠心室僵硬度的影响。
简介
高盐摄入可导致心脏肥大、胶原积累和舒张功能障碍。这些作用部分是由心脏肾素-血管紧张素-醛固酮系统的激活介导的。
方法
雄性自发性高血压大鼠（SHR，32 周）给予饮用水（SHR）、1%NaCl 溶液（SHR-Salt）或 1%NaCl 溶液加每日皮下注射螺内酯（80mg·kg-1）（SHRSalt-S）。年龄匹配的正常血压 Wistar 大鼠作为对照。8 周后，动物麻醉并插管以评估左心室和动脉血压。心脏停搏后，通过主动脉将双腔导管插入左心室，以获得原位左心室压力-容积曲线。
结果
所有 SHR 组的血压彼此相似，但与正常血压对照组不同（Wistar=109±2；SHR=118±2；SHR-Salt=117±2；SHR-Salt-S=116±2mmHg；P<0.05）。盐超负荷增强了 SHR 观察到的心脏肥大，并被螺内酯减弱（Wistar=2.90±0.06；SHR=3.44±0.07；SHR-Salt=3.68±0.07；SHR-Salt-S=3.46±0.05mg/g；P<0.05）。左心室 dP/dt 评估的心肌松弛因盐超负荷而受损，并通过螺内酯改善（Wistar=-3698±92；SHR=-3729±125；SHR-Salt=-3342±80；SHR-Salt-S=-3647±104mmHg/s；P<0.05）。盐超负荷未改变心室僵硬度，但螺内酯治疗将心室僵硬度降低至正常血压对照组观察到的水平（Wistar=1.40±0.04；SHR=1.60±0.05；SHR-Salt=1.67±0.12；SHR-Salt-S=1.45±0.03mmHg/ml；P<0.05）。
结论
螺内酯可降低成年 SHR 高盐摄入引起的左心室肥大和心室僵硬度。
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