Anwar K, Nakakuki K, Imai H, Inuzuka M
FUKUI MED SCH,DEPT PATHOL,MATSUOKA,FUKUI 91011,JAPAN. FUKUI MED SCH,DEPT BIOCHEM,MATSUOKA,FUKUI 91011,JAPAN.
Int J Oncol. 1995 Aug;7(2):391-7. doi: 10.3892/ijo.7.2.391.
To clarify the role of human papillomavirus (HPV) and Epstein-Barr virus (EBV) infection in gastric carcinogenesis in relation to overexpression of mutated p53 anti-oncogene, we used PCR to amplify DNA sequences of these viruses and immunohistochemistry to detect p53 overexpression in formaline-fixed, paraffin embedded blocks including 12 normal gastric and 51 gastric carcinoma specimens. HPV and EBV DNA were found in 17% and 0% of normal gastric tissues and in 45% and 27% of gastric carcinoma specimens, respectively. p53 overexpression was shown in 37% of gastric carcinoma specimens only. HPV infection rate was significantly higher in stage I gastric carcinomas as compared with stage IV carcinomas (p<0.03). p53 overexpression was significantly increased in well-differentiated adenocarcinomas as compared with poorly differentiated carcinomas (p<0.01). The rates of both HPV infection and p53 overexpression were significantly higher in gastric carcinomas without vascular invasion than in those with the invasion (p<0.02). No correlation was found between p53 overexpression and/or the presence of viral DNA (HPV/EBV) in regard to the depth of invasion, lymph node involvement, distant metastasis, and the location of the tumors. Our results suggest that some of the gastric carcinomas are associated with HPV and/or EBV infection and p53 mutations, and that all of these may be involved in the early stage of this malignancy. There was no correlation between HPV and or EBV infection and overexpression of p53 in gastric carcinoma.
为阐明人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）感染在胃癌发生中与突变型p53抗癌基因过表达的关系，我们采用聚合酶链反应（PCR）扩增这些病毒的DNA序列，并运用免疫组织化学方法检测12例正常胃组织和51例胃癌标本的福尔马林固定石蜡包埋块中p53的过表达情况。HPV和EBV DNA分别在17%的正常胃组织和45%的胃癌标本中被发现，而在正常胃组织和胃癌标本中的检出率分别为0%和27%。仅在37%的胃癌标本中显示有p53过表达。I期胃癌的HPV感染率显著高于IV期胃癌（p<0.03）。高分化腺癌的p53过表达显著高于低分化腺癌（p<0.01）。无血管侵犯的胃癌的HPV感染率和p53过表达率均显著高于有血管侵犯的胃癌（p<0.02）。在浸润深度、淋巴结受累情况、远处转移及肿瘤位置方面，未发现p53过表达和/或病毒DNA（HPV/EBV）的存在之间存在相关性。我们的结果表明，部分胃癌与HPV和/或EBV感染及p53突变有关，且所有这些因素可能均参与了该恶性肿瘤的早期阶段。胃癌中HPV和/或EBV感染与p53过表达之间无相关性。
