Snietura Miroslaw, Waniczek Dariusz, Piglowski Wojciech, Kopec Agnieszka, Nowakowska-Zajdel Ewa, Lorenc Zbigniew, Muc-Wierzgon Malgorzata
Miroslaw Snietura, Wojciech Piglowski, Agnieszka Kopec, Tumor Pathology Department, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, 44-101 Gliwice, Poland.
World J Gastroenterol. 2014 Jun 7;20(21):6632-7. doi: 10.3748/wjg.v20.i21.6632.
To demonstrate the presence and biological activity of human papilloma virus (HPV) in gastric cancer (GAC) tissues.
The study involved 84 surgically treated patients with gastric adenocarcinoma, regardless of the clinical stage of the disease. The presence of HPV DNA of high oncogenic risk types in formalin-fixed, paraffin-embedded tumor samples was determined using quantitative polymerase chain reaction analysis. A stringent protocol of prevention of cross- and environmental contamination was applied during DNA isolation, and amplification, as well as confirmation of the biological activity of the virus in tumor cells, was implemented. The study utilized the Real-time High Risk HPV test, which detects the DNA of 14 HPV subtypes that are considered to have high oncogenic potential. The overexpression of the p16(INK4a) protein assessed immunohistochemically was considered confirmation of the HPV infection.
Among the 89 patients initially included in the study group, diagnostic results were obtained for 84 individuals. In five cases, either the histopathological material was too scant to isolate the necessary amount of DNA, or the isolated DNA was significantly degraded, resulting in the failure of internal control amplification within the predefined number of 35 cycles. Those patients were excluded from further analysis. The amplification of HPV DNA was demonstrated in none of the 84 tissue samples; thus, all cases were considered to have a negative DNA status of highly oncogenic HPV subtypes. Immunohistochemical staining provided diagnostic results for all of the examined tissue samples, and excluded the accumulation of the p16(INK4a) protein in tumor cells, thus confirming the lack of active HPV infection in all of the individuals.
The study does not confirm the presence or biological activity of HPV in tumor tissues. Thus, the relationship between GAC and HPV infection, in the Central European population seems doubtful.
证实人乳头瘤病毒（HPV）在胃癌（GAC）组织中的存在及生物学活性。
该研究纳入84例接受手术治疗的胃腺癌患者，不考虑疾病的临床分期。采用定量聚合酶链反应分析，测定福尔马林固定、石蜡包埋肿瘤样本中高致癌风险型HPV DNA的存在情况。在DNA分离、扩增过程中，采用严格的预防交叉污染和环境污染方案，并对肿瘤细胞中病毒的生物学活性进行确认。该研究使用实时高危HPV检测，可检测14种被认为具有高致癌潜力的HPV亚型的DNA。免疫组织化学评估的p16（INK4a）蛋白过表达被视为HPV感染的确认。
在最初纳入研究组的89例患者中，84例获得诊断结果。5例中，要么组织病理学材料过少，无法分离出足够量的DNA，要么分离出的DNA严重降解，导致在预定的35个循环内内部对照扩增失败。这些患者被排除在进一步分析之外。84个组织样本中均未检测到HPV DNA的扩增；因此，所有病例均被认为高致癌HPV亚型的DNA状态为阴性。免疫组织化学染色为所有检测的组织样本提供了诊断结果，并排除了肿瘤细胞中p16（INK4a）蛋白的积累，从而证实所有个体均无活跃的HPV感染。
该研究未证实在肿瘤组织中存在HPV或其生物学活性。因此，在中欧人群中，GAC与HPV感染之间的关系似乎存疑。
