Bragadottir G, Eiriksdottir G, Sigurdsson A, Barkardottir R, Gudmundsson J, Jonasson J, Ingvarsson S
UNIV HOSP ICELAND,DEPT PATHOL,IS-121 REYKJAVIK,ICELAND.
Int J Oncol. 1995 Oct;7(4):871-6. doi: 10.3892/ijo.7.4.871.
Several chromosome regions exhibit loss of heterozygosity (LOH) in human breast carcinoma and are thought to carry tumor suppressor genes. We have analysed human breast tumors with 9 polymorphic microsatellite markers that are specific to chromosome 6q. The mapping of smallest region of overlap (SRO) indicated location of candidate suppressor genes at 6q23 and 6q27. Variations in estrogen receptor (ER) expression were independent of the number of copies of the corresponding gene. Tumors with and without LOH on chromosome 6q were tested for association with clinicopathological factors. A significant association was found between LOH at 6q and the following: high S-phase, aneuploidy, deletions at chromosomes 3p and 9p and lower survival rate. In a multivariate model LOH at 6q is an independent prognostic variable and patients having tumors with LOH have approximately twofold increase in relative risk of death. It can be concluded that the 6q deletions give additional prognostic information that might be useful in breast cancer treatment.
在人类乳腺癌中，几个染色体区域表现出杂合性缺失（LOH），并被认为携带着肿瘤抑制基因。我们用9个对6号染色体长臂特异的多态微卫星标记分析了人类乳腺肿瘤。最小重叠区域（SRO）的定位表明候选抑制基因位于6q23和6q27。雌激素受体（ER）表达的变化与相应基因的拷贝数无关。对6号染色体长臂有和没有LOH的肿瘤进行了与临床病理因素的相关性检测。发现6号染色体长臂的LOH与以下因素之间存在显著相关性：高S期、非整倍体、3号染色体短臂和9号染色体短臂的缺失以及较低的生存率。在多变量模型中，6号染色体长臂的LOH是一个独立的预后变量，患有具有LOH肿瘤的患者死亡相对风险增加约两倍。可以得出结论，6号染色体长臂的缺失提供了可能对乳腺癌治疗有用的额外预后信息。
