• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

激酶 DYRKIA 通过磷酸化剪接体成分 SAP155 来调节精原细胞中的前体 mRNA 剪接以及精原细胞和支持细胞的增殖，这一过程发生在出生后小鼠的睾丸中。

The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes.

机构信息

Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.

出版信息

Mol Cell Biochem. 2011 Sep;355(1-2):217-22. doi: 10.1007/s11010-011-0857-7. Epub 2011 May 8.

DOI:10.1007/s11010-011-0857-7
PMID:21553260
Abstract

SAP155 is an essential component of the spliceosome and its phosphorylation is required for splicing catalysis, but little is known concerning its function and regulation during spermatogenesis in postnatal murine testes. We report that inhibition of dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) IA strongly suppressed the mitogen-stimulated SAP155 phosphorylation and constitutive splicing of IκB pre-mRNA as well as the proliferation of spermatogonial and Sertoli cells in cultures of the 6-day post partum testes and a spermatogonial cell line, but not in a Sertoli cell line. Our findings suggest that the active spliceosome, containing SAP155 phosphorylated by DYRKIA, performs pre-mRNA splicing in spermatogonia during testicular development.

摘要

SAP155 是剪接体的必需组成部分，其磷酸化对于剪接催化是必需的，但对于其在出生后小鼠睾丸中的精子发生过程中的功能和调节知之甚少。我们报告称，双重特异性酪氨酸磷酸化调节激酶（DYRK）IA 的抑制强烈抑制了有丝分裂原刺激的 SAP155 磷酸化和 IκB 前体 mRNA 的组成性剪接，以及产后 6 天睾丸和精原细胞系中精原细胞和支持细胞的增殖，但在支持细胞系中没有。我们的发现表明，含有 DYRKIA 磷酸化的 SAP155 的活性剪接体在睾丸发育过程中的精原细胞中进行前体 mRNA 剪接。

相似文献

1
The kinase DYRKIA regulates pre-mRNA splicing in spermatogonia and proliferation of spermatogonia and Sertoli cells by phosphorylating a spliceosomal component, SAP155, in postnatal murine testes.激酶 DYRKIA 通过磷酸化剪接体成分 SAP155 来调节精原细胞中的前体 mRNA 剪接以及精原细胞和支持细胞的增殖，这一过程发生在出生后小鼠的睾丸中。
Mol Cell Biochem. 2011 Sep;355(1-2):217-22. doi: 10.1007/s11010-011-0857-7. Epub 2011 May 8.
2
Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes.磷酸化 SAP155，剪接体成分，定位于出生后小鼠睾丸的染色质中。
Biochem Biophys Res Commun. 2010 Mar 19;393(4):577-81. doi: 10.1016/j.bbrc.2010.02.014. Epub 2010 Feb 12.
3
Interactions between SAP155 and FUSE-binding protein-interacting repressor bridges c-Myc and P27Kip1 expression.SAP155 与 FUSE 结合蛋白相互作用的阻遏蛋白相互作用，连接 c-Myc 和 P27Kip1 的表达。
Mol Cancer Res. 2013 Jul;11(7):689-98. doi: 10.1158/1541-7786.MCR-12-0673. Epub 2013 Apr 17.
4
Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155).蛋白磷酸酶-1的核抑制剂（NIPP1）引导蛋白磷酸酶-1（PP1）使U2小核核糖核蛋白颗粒（snRNP）组分、剪接体相关蛋白155（Sap155）去磷酸化。
J Biol Chem. 2008 Dec 19;283(51):35805-14. doi: 10.1074/jbc.M805468200. Epub 2008 Oct 8.
5
The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site.蛋白激酶DYRK1A在苏氨酸434位点使剪接因子SF3b1/SAP155磷酸化，这是一个新发现的体内磷酸化位点。
BMC Biochem. 2006 Mar 2;7:7. doi: 10.1186/1471-2091-7-7.
6
Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1.剪接因子SAP155和NIPP1之间的磷酸化依赖性相互作用。
J Biol Chem. 2002 Aug 30;277(35):31834-41. doi: 10.1074/jbc.M204427200. Epub 2002 Jun 24.
7
Cyclin E associates with components of the pre-mRNA splicing machinery in mammalian cells.细胞周期蛋白E在哺乳动物细胞中与前体mRNA剪接机制的组分相关联。
Mol Cell Biol. 1998 Aug;18(8):4526-36. doi: 10.1128/MCB.18.8.4526.
8
CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1.CDK11 通过磷酸化 SF3B1 调控前体 mRNA 的剪接。
Nature. 2022 Sep;609(7928):829-834. doi: 10.1038/s41586-022-05204-z. Epub 2022 Sep 14.
9
Spliceostatin A inhibits spliceosome assembly subsequent to prespliceosome formation.拼接抑素 A 抑制剪接体复合物的组装，其作用发生在预剪接体形成之后。
Nucleic Acids Res. 2010 Oct;38(19):6664-72. doi: 10.1093/nar/gkq494. Epub 2010 Jun 6.
10
Specific inhibition of serine- and arginine-rich splicing factors phosphorylation, spliceosome assembly, and splicing by the antitumor drug NB-506.抗肿瘤药物NB-506对富含丝氨酸和精氨酸的剪接因子磷酸化、剪接体组装及剪接的特异性抑制作用
Cancer Res. 2001 Sep 15;61(18):6876-84.

引用本文的文献

1
The "Janus-like" RNA-editing machinery in innate antiviral immunity.天然抗病毒免疫中的“类两面神”RNA编辑机制
Curr Trends Immunol. 2022;23:23-32.
2
Identification of Nrl1 Domains Responsible for Interactions with RNA-Processing Factors and Regulation of Nrl1 Function by Phosphorylation.鉴定 Nrl1 结构域负责与 RNA 加工因子相互作用及磷酸化调控 Nrl1 功能。
Int J Mol Sci. 2021 Jun 29;22(13):7011. doi: 10.3390/ijms22137011.
3
Novel targets identified by integrated proteomic and phosphoproteomic analysis in spermatogenesis of swamp buffalo (Bubalus bubalis).

本文引用的文献

1
Phosphorylated SAP155, the spliceosomal component, is localized to chromatin in postnatal mouse testes.磷酸化 SAP155，剪接体成分，定位于出生后小鼠睾丸的染色质中。
Biochem Biophys Res Commun. 2010 Mar 19;393(4):577-81. doi: 10.1016/j.bbrc.2010.02.014. Epub 2010 Feb 12.
2
Reduced expression of an RNA-binding protein by prolactin leads to translational silencing of programmed cell death protein 4 and apoptosis in newt spermatogonia.催乳素导致RNA结合蛋白表达降低，进而使蝾螈精原细胞中的程序性细胞死亡蛋白4发生翻译沉默并引发细胞凋亡。
J Biol Chem. 2009 Aug 28;284(35):23260-71. doi: 10.1074/jbc.M109.018622. Epub 2009 Jun 25.
3
综合蛋白质组学和磷酸化蛋白质组学分析鉴定的沼泽水牛（Bubalus bubalis）精子发生中的新靶标。
Sci Rep. 2020 Sep 24;10(1):15659. doi: 10.1038/s41598-020-72353-4.
4
Splicing Factor 3B Subunit 1 Interacts with HIV Tat and Plays a Role in Viral Transcription and Reactivation from Latency.剪接因子 3B 亚基 1 与 HIV Tat 相互作用，并在潜伏病毒转录和激活中发挥作用。
mBio. 2018 Nov 6;9(6):e01423-18. doi: 10.1128/mBio.01423-18.
5
Glycogen synthase kinase-3 and alternative splicing.糖原合成酶激酶-3 和选择性剪接。
Wiley Interdiscip Rev RNA. 2018 Nov;9(6):e1501. doi: 10.1002/wrna.1501. Epub 2018 Aug 17.
6
Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin.细胞周期蛋白依赖性激酶 1（CDK1）和 CDK2 在调节剪接因子 3B1 与染色质相互作用方面具有相反的作用。
J Biol Chem. 2018 Jun 29;293(26):10220-10234. doi: 10.1074/jbc.RA118.001654. Epub 2018 May 15.
7
Melanoma Differentiation-associated Gene 7/IL-24 Exerts Cytotoxic Effects by Altering the Alternative Splicing of Bcl-x Pre-mRNA via the SRC/PKCδ Signaling Axis.黑色素瘤分化相关基因7/白细胞介素-24通过SRC/PKCδ信号轴改变Bcl-x前体mRNA的可变剪接发挥细胞毒性作用。
J Biol Chem. 2016 Oct 7;291(41):21669-21681. doi: 10.1074/jbc.M116.737569. Epub 2016 Aug 12.
8
Major spliceosome defects cause male infertility and are associated with nonobstructive azoospermia in humans.主要剪接体缺陷会导致男性不育，并且与人类非梗阻性无精子症相关。
Proc Natl Acad Sci U S A. 2016 Apr 12;113(15):4134-9. doi: 10.1073/pnas.1513682113. Epub 2016 Mar 28.
Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA.
剪接抑制素A作用于剪接因子3b（SF3b），并抑制前体信使核糖核酸（pre-mRNA）的剪接和核滞留。
Nat Chem Biol. 2007 Sep;3(9):576-83. doi: 10.1038/nchembio.2007.18. Epub 2007 Jul 22.
4
NSSR1 is regulated in testes development and cryptorchidism and promotes the exon 5-included splicing of CREB transcripts.NSSR1在睾丸发育和隐睾症中受到调控，并促进包含CREB转录本外显子5的剪接。
Mol Reprod Dev. 2007 Nov;74(11):1363-72. doi: 10.1002/mrd.20719.
5
The expression of the testis-specific Dyrk4 kinase is highly restricted to step 8 spermatids but is not required for male fertility in mice.睾丸特异性Dyrk4激酶的表达高度局限于第8期精子细胞，但对小鼠雄性生育力并非必需。
Mol Cell Endocrinol. 2007 Mar 15;267(1-2):80-8. doi: 10.1016/j.mce.2006.12.041. Epub 2007 Jan 17.
6
The protein kinase DYRK1A phosphorylates the splicing factor SF3b1/SAP155 at Thr434, a novel in vivo phosphorylation site.蛋白激酶DYRK1A在苏氨酸434位点使剪接因子SF3b1/SAP155磷酸化，这是一个新发现的体内磷酸化位点。
BMC Biochem. 2006 Mar 2;7:7. doi: 10.1186/1471-2091-7-7.
7
Spermatogonial cell-mediated activation of an IkappaBzeta-independent nuclear factor-kappaB pathway in Sertoli cells induces transcription of the lipocalin-2 gene.精原细胞介导的支持细胞中不依赖IκBζ的核因子κB信号通路激活可诱导脂质运载蛋白-2基因转录。
Mol Endocrinol. 2006 Apr;20(4):904-15. doi: 10.1210/me.2005-0423. Epub 2005 Dec 1.
8
TESSP-1: a novel serine protease gene expressed in the spermatogonia and spermatocytes of adult mouse testes.TESSP-1：一个在成年小鼠睾丸的精原细胞和精母细胞中表达的新型丝氨酸蛋白酶基因。
Mol Reprod Dev. 2005 Jan;70(1):1-10. doi: 10.1002/mrd.20184.
9
Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors.细胞周期蛋白L2的特性：一种具有富含精氨酸/丝氨酸结构域的新型细胞周期蛋白，受DYRK1A磷酸化并与剪接因子共定位
J Biol Chem. 2004 Feb 6;279(6):4612-24. doi: 10.1074/jbc.M310794200. Epub 2003 Nov 17.
10
Alternative splicing in the testes.睾丸中的可变剪接。
Curr Opin Genet Dev. 2002 Oct;12(5):615-19. doi: 10.1016/s0959-437x(02)00347-7.