[利用原子力显微镜分析猪前体脂肪细胞分化]

[Analysis of porcine preadipocytes differentiation by atomic force microscope].

作者信息

Li Shengpu, Shi Ruyi, Wang Qiulan, Wang Mu, Gan Rui, Pan Jie, Cai Jiye, Zhang Shouquan

机构信息

Department of Chemistry, Jinan University, Guangzhou 510632, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2011 Jan;27(1):124-30.

PMID:21553498

Abstract

Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.

摘要

脂肪形成过程中的异常变化与许多疾病的发生密切相关。为了了解脂肪的形成机制，我们使用原子力显微镜（AFM）对猪前脂肪细胞分化过程中的形态和力学性能进行了表征。前脂肪细胞和脂肪细胞在形态上有所不同。通过超微结构检测发现，脂肪细胞的表面粗糙度小于前脂肪细胞。基于AFM的力谱分析表明，前脂肪细胞在分化过程中力学性能发生了变化。前脂肪细胞的粘附力、硬度和杨氏模量比脂肪细胞高20%。因此，AFM对与脂肪细胞形成相关的膜变化分析提供了纳米级别的定量数据，以进一步研究脂肪细胞的形成机制。