Xiong F, Mi Z, Gu N
State Key Laboratory of Bioelectronics, Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China.
Pharmazie. 2011 Mar;66(3):158-64.
As it has been generally reported that oppositely charged cationic liposomes (CLs) are superior to either neutral or anionic liposomes as gene delivery carrier, interest in the properties, structures, transfection mechanism of CLs and so forth arises unprecedentedly. However, our understanding about the mechanism of CLs-gene complexes (lipoplex)-cell interaction and factors influencing the transfection efficiency (TE) of CLs remains poor. In this article, we describe some new results aimed at elucidating the relationship between the chemical-physical properties of lipoplex with TE and introducing recent applications of CLs in gene therapy.
一般报道称，带相反电荷的阳离子脂质体（CLs）作为基因传递载体优于中性或阴离子脂质体，因此对CLs的性质、结构、转染机制等的研究兴趣空前高涨。然而，我们对CLs-基因复合物（脂质体复合物）与细胞相互作用的机制以及影响CLs转染效率（TE）的因素仍知之甚少。在本文中，我们描述了一些旨在阐明脂质体复合物的化学物理性质与转染效率之间关系的新结果，并介绍了CLs在基因治疗中的最新应用。
