Departamento de Microbiología y Parasitología, Facultad de Biología-CIBUS e Instituto de Acuicultura, Universidad de Santiago de Compostela, Santiago de Compostela, Spain.
Lett Appl Microbiol. 2011 Jul;53(1):114-9. doi: 10.1111/j.1472-765X.2011.03080.x. Epub 2011 May 31.
Edwardsiella tarda is an important pathogen in aquaculture where it can cause serious losses. A rapid detection of it is vital to minimize the mortalities caused by this disease, and in this work, the effectiveness of the selective differential Edw. tarda medium (ET) was evaluated for the diagnosis of edwardsiellosis as well as for its possible use in epidemiological studies.
ET medium was evaluated in parallel with the commercial Salmonella-Shigella agar (SS), which is usually employed for the selective isolation of enteric bacilli. Moreover, two general media (TSA-1 and MA) were employed as a control. The results obtained showed that ET is distinctly selective for the isolation of Edw. tarda, allowing its recovery from mixed cultures and natural samples as a unique species. In contrast, although colonies of Edw. tarda could be clearly distinguishable in SS because of the appearance of a characteristic black centre, other enteric and nonenteric bacterial species were also able to grow on this medium.
We recommend ET agar as an useful medium for the primary isolation of Edw. tarda from aquaculture samples.
The results obtained support ET medium as the most appropriate to develop epidemiological studies of edwardsiellosis in aquaculture and permits an earlier diagnosis of this important disease.
迟缓爱德华氏菌是水产养殖中的一种重要病原体，可导致严重损失。快速检测对于最大限度地减少该疾病引起的死亡率至关重要，在这项工作中，评估了选择性差异爱德华氏菌培养基（ET）在诊断爱德华氏菌病以及在流行病学研究中的潜在用途的有效性。
平行评估了 ET 培养基与通常用于选择性分离肠杆菌的商业沙门氏菌-志贺氏菌琼脂（SS）。此外，还使用了两种普通培养基（TSA-1 和 MA）作为对照。结果表明，ET 培养基对迟缓爱德华氏菌的分离具有明显的选择性，可从混合培养物和自然样本中回收，作为唯一的物种。相比之下，尽管 SS 中爱德华氏菌的菌落由于出现特征性的黑色中心而可以清晰地区分，但其他肠内和非肠内细菌也可以在该培养基上生长。
我们建议 ET 琼脂作为从水产养殖样本中初步分离迟缓爱德华氏菌的有用培养基。
研究结果支持 ET 培养基最适合水产养殖中爱德华氏菌病的流行病学研究，并允许更早诊断这种重要疾病。
