Institute for Hormone and Fertility Research, Grandweg 64, 2000 Hamburg 54, FRG.
J Neuroendocrinol. 1992 Aug;4(4):505-13. doi: 10.1111/j.1365-2826.1992.tb00198.x.
cDNA clones corresponding to the vasotocin precursor polypeptide were isolated from a chicken hypothalamic library and sequenced. The derived amino-acid sequence indicates a precursor of comparable structural organization to that described for members of the vasotocin/vasopressin gene family from other species. Unlike in mammals the C-terminal glycopeptide moiety appears not be cleaved off from the neurophysin. Subsequent screening of a chicken genomic library permitted an analysis also of the vasotocin gene structure and exonic composition. The 5'region upstream of the first exon was sequenced and revealed an unusual pattern of 49 repetitive -YYCYCYAAAYY- motifs, together with a polyadenyl region supporting a bend in the DNA, and a long pyrimidine-rich sequence. Three AP2-like elements, identified in the mammalian vasopressin gene, were also observed in the immediate upstream region. There was no obvious homology to the promoter regions of the known oxytocin genes, nor to any other sequence deposited in available databases, nor to other known cis-elements.
从鸡下丘脑文库中分离出与加压素前体多肽相对应的 cDNA 克隆，并对其进行了测序。推导的氨基酸序列表明，该前体与来自其他物种的加压素/血管升压素基因家族的成员具有相似的结构组织。与哺乳动物不同，神经垂体中的 C 末端糖肽部分似乎没有从神经垂体中切割下来。随后对鸡基因组文库的筛选也允许分析加压素基因结构和外显子组成。对第一个外显子上游的 5'区域进行了测序，揭示了一种不寻常的 49 个重复 -YYCYCYAAAYY- 基序模式，以及一个支持 DNA 弯曲的多聚腺苷酸区域和一个长嘧啶丰富的序列。在哺乳动物血管升压素基因中鉴定出的三个 AP2 样元件也在紧邻上游区域观察到。与已知催产素基因的启动子区域没有明显的同源性，也与任何其他已在可用数据库中存储的序列或其他已知顺式元件没有同源性。
