Michel G, Chauvet J, Chauvet M T, Acher R
Laboratory of Biological Chemistry, University of Paris VI, France.
Biochem Biophys Res Commun. 1987 Dec 16;149(2):538-44. doi: 10.1016/0006-291x(87)90401-3.
Vasotocin-associated neurophysin (MSEL-neurophysin) from the frog Rana esculenta has been isolated and sequenced through tryptic and staphylococcal proteinase peptides and cyanogen bromide fragments. This protein appears homologous to the mammalian vasopressin-associated neurophysin with a C-terminal glycopeptide extension homologous to the mammalian copeptin. In contrast to the two-step processing of mammalian vasopressin/MSEL-neurophysin/copeptin precursor, a single cleavage is therefore involved in the processing of the amphibian vasotocin/neurophysin precursor. It appears that the physiological release of the vasopressin-like hormone from the N-terminal end of the protein precursor is not dependent upon a previous trimming of the C-terminal copeptin-like moiety.
食用蛙的血管紧张素相关神经垂体素（MSEL-神经垂体素）已通过胰蛋白酶、葡萄球菌蛋白酶肽和溴化氰片段进行分离和测序。该蛋白质似乎与哺乳动物的加压素相关神经垂体素同源，其C末端糖肽延伸与哺乳动物的 copeptin 同源。与哺乳动物加压素/MSEL-神经垂体素/copeptin 前体的两步加工不同，两栖动物血管紧张素/神经垂体素前体的加工只涉及一次切割。似乎蛋白质前体N末端的加压素样激素的生理释放并不依赖于C末端copeptin样部分的预先修剪。
