甲苯二异氰酸酯引起的血清白蛋白构象变化：豚鼠反复吸入的研究

Toluene diisocyanate-induced conformational changes of serum albumin: a study on repeated inhalations in guinea-pigs.

作者信息

Kochman S, Lefebvre S, Bernard J, Maujean A, Cazabat A, Lavaud F, Manfait M

机构信息

Laboratoire de Médecine Expérimentale, Faculté de Médecine, Centre Hospitalier Universitaire, Reims, France.

出版信息

Toxicol Lett. 1990 Feb;50(2-3):165-71. doi: 10.1016/0378-4274(90)90007-9.

DOI:10.1016/0378-4274(90)90007-9

PMID:2155482

Abstract

High responder lines of Hartley guinea-pigs were sensitized by repeated inhalations of toluene diisocyanate (TDI). After 3 weeks, we demonstrated a degree of TDI substitution of the serum-albumin-enriched fraction (AEF) and we ascertained the sensitization of the most exposed animals using PCA methodology. Fourier transform infrared spectroscopy (FT-IR), used to investigate conformational changes in AEF, highlighted the structural modifications of the native protein conformation. Such crucial changes may support, at least in part, the relationship between TDI exposure and triggering of hypersensitivity reactions.

摘要

通过反复吸入甲苯二异氰酸酯（TDI）使高反应性品系的哈特利豚鼠致敏。3周后，我们证实了血清白蛋白富集组分（AEF）中TDI的替代程度，并使用被动皮肤过敏试验（PCA）方法确定了暴露最严重动物的致敏情况。用于研究AEF构象变化的傅里叶变换红外光谱（FT-IR）突出了天然蛋白质构象的结构修饰。这些关键变化可能至少部分支持TDI暴露与超敏反应触发之间的关系。